Department of Preventive Dentistry, Osaka University Graduate School of Dentistry, Japan.
Joint Research Laboratory (SARAYA) for Advanced Oral Environmental Science, Osaka University Graduate School of Dentistry, Japan.
Arch Oral Biol. 2021 Apr;124:105058. doi: 10.1016/j.archoralbio.2021.105058. Epub 2021 Jan 23.
The purpose of this study was to examine whether curcumin, a turmeric root extract, protects human gingival epithelial (HGE) cells from the cytotoxic effects ofPorphyromonas gingivalis outer membrane vesicles (OMVs).
OMVs were prepared fromP. gingivalis OMZ314 and used to stimulate human gingival epithelial (HGE) cells. The effects of curcumin on cellular expression of inflammatory cytokines were evaluated using real-time reverse transcription-polymerase chain reaction assays, while those on cellular migration were examined with a scratch wound assay. Furthermore, HGE cells were incubated with OMVs in the presence or absence of curcumin, then intracellular invasion by OMVs was observed with confocal laser scanning microscopy. Also, the effects of curcumin on cellular apoptotic death was examined.
Gene expressions of IL-6, IL-1β, and TNF-α in HGE cells stimulated with OMVs were significantly suppressed by curcumin in a dose-dependent manner, with suppressed protein production also noted. Moreover, curcumin inhibited the cytotoxic effects of OMVs on cellular migration. Finally, curcumin inhibited OMV adherence to and entry of cells, as well as cellular apoptotic death in a dose-dependent manner.
Curcumin showed marked inhibitory effects against the cytotoxic actions of P. gingivalis OMVs, indicating possible potency for preventing periodontal disease.
本研究旨在探讨姜黄素（姜黄根提取物）是否能保护人牙龈上皮（HGE）细胞免受牙龈卟啉单胞菌外膜囊泡（OMV）的细胞毒性作用。
从 P. gingivalis OMZ314 中制备 OMV 并用于刺激人牙龈上皮（HGE）细胞。使用实时逆转录聚合酶链反应（RT-PCR）检测姜黄素对细胞炎症因子表达的影响，通过划痕伤口试验检测其对细胞迁移的影响。此外，在存在或不存在姜黄素的情况下将 HGE 细胞与 OMV 孵育，然后使用共聚焦激光扫描显微镜观察 OMV 的细胞内入侵。还检查了姜黄素对细胞凋亡的影响。
姜黄素以剂量依赖性方式显著抑制 OMV 刺激的 HGE 细胞中 IL-6、IL-1β 和 TNF-α 的基因表达，同时也观察到蛋白产物的减少。此外，姜黄素抑制了 OMV 对细胞迁移的细胞毒性作用。最后，姜黄素以剂量依赖性方式抑制 OMV 与细胞的黏附和进入，以及细胞凋亡。
姜黄素对牙龈卟啉单胞菌 OMV 的细胞毒性作用表现出显著的抑制作用，表明其可能具有预防牙周病的潜力。
