Birgen Cansu, Degnes Kristin F, Markussen Sidsel, Wentzel Alexander, Sletta Håvard
Department of Chemical Engineering, NTNU, 7491, Trondheim, Norway.
Department of Thermal Energy, SINTEF Energy Research, 7034, Trondheim, Norway.
Biotechnol Biofuels. 2021 Jan 30;14(1):34. doi: 10.1186/s13068-021-01886-1.
Butanol (n-butanol) has been gaining attention as a renewable energy carrier and an alternative biofuel with superior properties to the most widely used ethanol. We performed 48 anaerobic fermentations simultaneously with glucose and xylose as representative lignocellulosic sugars by Clostridium beijerinckii NCIMB 8052 in BioLector® microbioreactors to understand the effect of different sugar mixtures on fermentation and to demonstrate the applicability of the micro-cultivation system for high-throughput anaerobic cultivation studies. We then compared the results to those of similar cultures in serum flasks to provide insight into different setups and measurement methods.
ANOVA results showed that the glucose-to-xylose ratio affects both growth and production due to Carbon Catabolite Repression. The study demonstrated successful use of BioLector® system for the first time for screening several media and sugar compositions under anaerobic conditions by using online monitoring of cell mass and pH in real-time and at unprecedented time-resolution. Fermentation products possibly interfered with dissolved oxygen (DO) measurements, which require a careful interpretation of DO monitoring results.
The statistical approach to evaluate the microbioreactor setup, and information obtained in this study will support further research in bioreactor and bioprocess design, which are very important aspects of industrial fermentations of lignocellulosic biomass.
正丁醇作为一种可再生能源载体和替代生物燃料，因其性能优于最广泛使用的乙醇而受到关注。我们在BioLector®微生物反应器中，使用拜氏梭菌NCIMB 8052同时对葡萄糖和木糖这两种代表性木质纤维素糖进行了48次厌氧发酵，以了解不同糖混合物对发酵的影响，并证明该微培养系统在高通量厌氧培养研究中的适用性。然后，我们将结果与血清瓶中类似培养物的结果进行比较，以深入了解不同的设置和测量方法。
方差分析结果表明，由于碳分解代谢物阻遏，葡萄糖与木糖的比例会影响生长和产物生成。该研究首次证明了BioLector®系统在厌氧条件下通过实时、以前所未有的时间分辨率在线监测细胞质量和pH值来筛选多种培养基和糖组成方面的成功应用。发酵产物可能会干扰溶解氧（DO）测量，这需要对DO监测结果进行仔细解读。
评估微生物反应器设置的统计方法以及本研究中获得的信息将支持生物反应器和生物工艺设计的进一步研究，这是木质纤维素生物质工业发酵的非常重要的方面。
