College of Life Sciences, Sichuan Normal University, Chengdu, 610101, China.
Key Laboratory of Synthetic Biology, CAS Center for Excellence in Molecular Plant Sciences, Chinese Academy of Sciences, 300 Fenglin Road, Shanghai, 200032, China.
World J Microbiol Biotechnol. 2021 Oct 26;37(12):205. doi: 10.1007/s11274-021-03173-5.
n-Butanol is an essential chemical intermediate produced through microbial fermentation. However, its toxicity to microbial cells has limited its production to a great extent. The anaerobe lactic acid bacteria (LAB) are the most resistant to n-butanol, so it should be the first choice for improving n-butanol production. The present article aims to review the following aspects of n-butanol production by LAB: (1) the tolerance of LAB to n-butanol, including its tolerance level and potential tolerance mechanisms; (2) genome editing tools in the n-butanol-resistant LAB; (3) methods of LAB modification for n-butanol production and the production levels after modification. This review will provide a theoretical basis for further research on n-butanol production by LAB.
正丁醇是一种通过微生物发酵生产的重要化学中间体。然而，其对微生物细胞的毒性在很大程度上限制了它的生产。厌氧菌乳酸菌（LAB）对正丁醇的抗性最强，因此应该是提高正丁醇产量的首选。本文旨在综述 LAB 生产正丁醇的以下几个方面：（1）LAB 对正丁醇的耐受性，包括其耐受水平和潜在的耐受机制；（2）耐正丁醇 LAB 的基因组编辑工具；（3）LAB 改良生产正丁醇的方法及改良后的生产水平。本综述将为进一步研究 LAB 生产正丁醇提供理论基础。
