School of Biomedical Sciences, Charles Sturt University, Wagga Wagga 2650, New South Wales, Australia.
The University of Queensland, Centre for Animal Science, Queensland Alliance for Agriculture and Food Innovation, Brisbane 4072, Queensland, Australia.
Poult Sci. 2021 Mar;100(3):100891. doi: 10.1016/j.psj.2020.12.004. Epub 2020 Dec 10.
Campylobacter spp. contaminated poultry products are strongly associated with foodborne illnesses worldwide. Development of effective management strategies to reduce contamination by Campylobacter spp. requires an improved understanding of the numerous factors that drive these contamination processes. Currently, chicken farms are using more free-range chicken meat production systems in response to consumer preferences. However, Campylobacter spp. colonization has rarely been investigated on free-range broiler farms. The present study investigated the temporal and environmental factors influencing Campylobacter spp. colonization of free-range broilers as well as potential sources and genetic diversity of Campylobacter jejuni (C. jejuni) and Campylobacter coli (C. coli) in commercial free-range broiler farms. Genetic linkages among the isolates were analyzed using flaA amplicon analysis. Campylobacter coli was first detected in fecal samples of a commercial free-range broiler flock on day 10 of rearing. Multiple genotypes of C. jejuni and C. coli were identified in this study. The farm environment was identified as a potential source of C. jejuni and C. coli colonization of free-range broilers. The dominant Campylobacter genotype varied between free-range broiler farms over time, with C. jejuni being the most frequently isolated species. These findings enhance the understanding of C. jejuni and C. coli colonization in free-range broiler farms and could inform the development of more effective intervention strategies to help control this important foodborne pathogen.
弯曲菌属污染的家禽产品与全球食源性疾病密切相关。为了开发有效的管理策略来减少弯曲菌属的污染，需要更好地了解驱动这些污染过程的众多因素。目前，为了满足消费者的偏好，养鸡场正在更多地采用散养鸡肉生产系统。然而，弯曲菌属在散养肉鸡场的定植情况很少被研究。本研究调查了影响散养肉鸡弯曲菌属定植的时间和环境因素，以及商业散养肉鸡场中空肠弯曲菌（C. jejuni）和结肠弯曲菌（C. coli）的潜在来源和遗传多样性。使用 flaA 扩增子分析分析了分离株之间的遗传联系。在饲养的第 10 天，首次在商业散养肉鸡群的粪便样本中检测到结肠弯曲菌。本研究鉴定出多种空肠弯曲菌和结肠弯曲菌的基因型。农场环境被确定为散养肉鸡弯曲菌定植的潜在来源。随着时间的推移，不同散养肉鸡场的优势弯曲菌基因型发生变化，空肠弯曲菌是最常分离到的物种。这些发现增强了对散养肉鸡场中空肠弯曲菌和结肠弯曲菌定植的理解，并为开发更有效的干预策略以帮助控制这一重要的食源性病原体提供了信息。
