通过对甘蓝型油菜两个亲本的全基因组重测序鉴定与钾利用效率相关的 QTLs 及其潜在的候选基因。

Identification of QTLs associated with potassium use efficiency and underlying candidate genes by whole-genome resequencing of two parental lines in Brassica napus.

机构信息

National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, China; Microelement Research Centre, Key Laboratory of Arable Land Conservation (Middle and Lower Reaches of Yangtze River), Ministry of Agriculture and Rural Affairs, Huazhong Agricultural University, Wuhan 430070, China.

National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, China; The James Hutton Institute, Invergowrie, Dundee DD2 5DA, UK; Distinguished Scientist Fellowship Program, King Saud University, Riyadh 11451, Saudi Arabia.

出版信息

Genomics. 2021 Mar;113(2):755-768. doi: 10.1016/j.ygeno.2021.01.020. Epub 2021 Jan 29.

DOI:10.1016/j.ygeno.2021.01.020

PMID:33516850

Abstract

Breeding crops that acquire and/or utilize potassium (K) more effectively could reduce the use of K fertilizers. Sixteen traits affecting K use efficiency (KUE) at the seedling stage were investigated in a B. napus double haploid population grown at an optimal K supply (OK) and a low K supply (LK) in a hydroponic culture system. In total, 50 and 62 QTLs associated with these traits were identified at OK and LK, respectively. A total of 25 orthologues of 23 Arabidopsis genes regulating K transport were identified in the confidence intervals of nine QTLs impacting shoot dry weight at LK, and 22 of these showed variations in coding sequences and/or exhibited significant differences in mRNA abundances in roots at LK between the two parental lines. This study provided insights to the genetic basis of KUE in B. napus, which will accelerate the breeding of K-efficient rapeseed cultivars by marker-assisted selection.

摘要

培育能够更有效地吸收和/或利用钾（K）的作物可以减少 K 肥料的使用。在水培培养系统中，在最佳 K 供应（OK）和低 K 供应（LK）下，对甘蓝型油菜双单倍体群体的 16 个影响 K 使用效率（KUE）的特性进行了研究。总共在 OK 和 LK 条件下分别鉴定到与这些性状相关的 50 个和 62 个 QTL。在影响 LK 条件下地上部干重的 9 个 QTL 的置信区间中，共鉴定到 23 个调控 K 转运的拟南芥基因的 25 个直系同源物，其中 22 个在两个亲本系之间在编码序列中存在差异，或在 LK 条件下根部的 mRNA 丰度存在显著差异。本研究为油菜 KUE 的遗传基础提供了见解，这将通过标记辅助选择加速高效 K 油菜品种的培育。