Department of Clinical Biochemistry, Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
Department of Hematology-Oncology, Faculty of Medicine, Firoozgar Clinical Research Development Center, Iran University of Medical Sciences, Tehran, Iran.
Turk J Med Sci. 2021 Jun;51(3):1345-1353. doi: 10.3906/sag-2008-292. Epub 2021 Jun 28.
Adipocytokines, adipose tissue-derived proteins, were demonstrated to be involved in the pathogenesis of breast cancer. We assessed the mRNA expression of resistin, tumor necrosis factor-alpha (TNF-α), interleukins 6 and 8 (IL-6, and IL-8), and estrogen receptor alpha (ER-α) in peripheral blood mononuclear cells (PBMCs) of women with and without breast cancer.
The PBMCs were isolated from the whole blood of 32 women with breast cancer and 18 women without breast cancer using density gradient centrifugation. The mRNA expression of the target genes was measured by reverse-transcription polymerase chain reaction (RT-PCR). Body mass index was calculated, additionally, clinicopathological characteristics of the breast cancer patients were determined by histopathological examination.
The mRNA expression of resistin (3.5-fold) and IL-6 (15-fold) in PBMCs of breast cancer patients significantly increased in comparison to healthy controls. Resistin expression was significantly associated with inflammatory markers including TNF-α, IL-6, IL-8, but not with anthropometric indices. Logistic regression analysis revealed the studied adipokines were not associated with breast cancer. Based on the ROC curve analysis the diagnostic performance of IL-6 was significant (0.825, 95% CI: 0.549-0.94, p = 0.030), thus, it might be considered as a breast cancer biomarker that reflecting an early and inflammatory stage of the disease.
Breast cancer is not associated with increased expression of inflammatory cytokines in PBMCs. Our results suggested that a PBMC-based gene expression test may be developed to detect breast cancer early.
脂肪细胞因子是脂肪组织衍生的蛋白质，被证明与乳腺癌的发病机制有关。我们评估了乳腺癌患者和无乳腺癌患者外周血单个核细胞（PBMC）中抵抗素、肿瘤坏死因子-α（TNF-α）、白细胞介素 6 和 8（IL-6 和 IL-8）以及雌激素受体-α（ER-α）的 mRNA 表达。
使用密度梯度离心法从 32 名乳腺癌女性和 18 名无乳腺癌女性的全血中分离 PBMC。通过逆转录聚合酶链反应（RT-PCR）测量靶基因的 mRNA 表达。计算体重指数，此外，通过组织病理学检查确定乳腺癌患者的临床病理特征。
与健康对照组相比，乳腺癌患者 PBMC 中的抵抗素（3.5 倍）和 IL-6（15 倍）mRNA 表达显着增加。抵抗素的表达与 TNF-α、IL-6、IL-8 等炎症标志物显着相关，但与人体测量指数无关。Logistic 回归分析显示，所研究的脂肪因子与乳腺癌无关。基于 ROC 曲线分析，IL-6 的诊断性能具有显着意义（0.825，95%CI：0.549-0.94，p=0.030），因此，它可能被认为是一种乳腺癌生物标志物，反映了疾病的早期和炎症阶段。
乳腺癌与 PBMC 中促炎细胞因子的表达增加无关。我们的结果表明，可能开发基于 PBMC 的基因表达测试来早期检测乳腺癌。
