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采用即时检测和标准湿式化学分析仪对圈养美洲火烈鸟的色素血浆样本进行配对生化分析。

PAIRED BIOCHEMICAL ANALYSIS OF PIGMENTED PLASMA SAMPLES FROM ZOO-KEPT AMERICAN FLAMINGOS () USING A POINT-OF-CARE AND A STANDARD WET CHEMISTRY ANALYZER.

机构信息

The Exotic Clinic, Tel-Aviv 6918705, Israel,

The Department of Clinical Sciences, College of Veterinary Medicine, Kansas State University, Manhattan, KS 66506, USA.

出版信息

J Zoo Wildl Med. 2019 Sep;50(3):619-626. doi: 10.1638/2017-0110.

DOI:10.1638/2017-0110
PMID:33517631
Abstract

American flamingos () are commonly kept in zoological collections, making health monitoring essential. Use of point-of-care (POC) blood analyzers that require small volumes of whole blood samples produces prompt results allowing for rapid clinical decision-making. To evaluate and compare blood biochemistry analysis results analyzed by a POC biochemistry analyzer and a laboratory wet biochemistry analyzer, blood was collected from 17 apparently healthy zoo-kept American flamingos. Analyzer agreement was investigated using the Passing-Bablock regression analysis and Spearman correlation coefficients. Plasma samples from all birds were bright yellow in color. The results from the POC analyzer used in this study were found to be outside acceptance and clinical allowable error limits when compared with the laboratory analyzer for phosphorus (Phos), total protein (TP), albumin (Alb), glucose (Glu), creatine kinase (CK), and potassium (K). For aspartate aminotransferase (AST), results were within clinical allowable error but outside the acceptance limits, and for calcium (Ca) and sodium (Na), results were within both limits. The POC analyzer failed to measure the uric acid (UA) concentrations of all the samples, and reported all bile acids (BA) concentrations as below its minimal measurable limit. The use of analyzer-specific reference intervals is recommended for most analytes tested. The POC analyzer used in this study cannot be recommended for measuring UA concentrations in brightly colored samples from American flamingos.

摘要

美洲火烈鸟()通常被饲养在动物园中,因此对其健康监测至关重要。使用需要少量全血样本的即时检测(POC)血液分析仪可以快速获得结果,从而快速做出临床决策。为了评估和比较由 POC 生化分析仪和实验室湿式生化分析仪分析的血液生化分析结果,从 17 只明显健康的动物园饲养的美洲火烈鸟中采集了血液。使用 Passing-Bablock 回归分析和 Spearman 相关系数研究了分析仪的一致性。所有鸟类的血浆样本均呈鲜黄色。与实验室分析仪相比,本研究中使用的 POC 分析仪的磷(Phos)、总蛋白(TP)、白蛋白(Alb)、葡萄糖(Glu)、肌酸激酶(CK)和钾(K)的结果超出了可接受和临床允许的误差范围。对于天冬氨酸氨基转移酶(AST),结果在临床允许的误差范围内,但超出了可接受的范围,而对于钙(Ca)和钠(Na),结果在两个范围内。POC 分析仪无法测量所有样本的尿酸(UA)浓度,并报告所有胆汁酸(BA)浓度均低于其最小可测量限。建议对大多数检测的分析物使用特定于分析仪的参考区间。本研究中使用的 POC 分析仪不建议用于测量颜色鲜艳的美洲火烈鸟样本中的 UA 浓度。

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