Wang Q, Wang X F, Xing T, Li J L, Zhu X D, Zhang L, Gao F
College of Animal Science and Technology, Jiangsu Provincial Key Laboratory of Animal Origin Food Production and Safety Guarantee, Jiangsu Provincial Collaborative Innovation Center of Meat Production and Processing, Quality and Safety Control, Joint International Research Laboratory of Animal Health and Food Safety, National Experimental Teaching Demonstration Center of Animal Science, Nanjing Agricultural University, Nanjing 210095, China.
College of Science, Nanjing Agricultural University, Nanjing 210095, China.
Poult Sci. 2021 Mar;100(3):100909. doi: 10.1016/j.psj.2020.11.075. Epub 2020 Dec 9.
This study was conducted to investigate the individual and combined effects of xylo-oligosaccharides (XOS) and gamma-irradiated Astragalus polysaccharides (IAPS) on the growth performance and intestinal mucosal barrier function of broiler chickens. A total of 240 1-day-old Ross-308 chicks were allocated into 5 groups for 21 d: control group (basal diet), antibiotic growth promoter (AGP) group (basal diet supplemented with 50 mg/kg chlortetracycline), XOS group (basal diet supplemented with 100 mg/kg XOS), IAPS group (basal diet supplemented with 600 mg/kg IAPS), and XOS + IAPS group (basal diet supplemented with 100 mg/kg XOS and 600 mg/kg IAPS). The results showed that birds in the XOS + IAPS group showed higher ADG and lower feed-to-gain ratio than those in the control group (P < 0.05). The XOS, IAPS, and XOS + IASP treatments significantly increased villus height (VH) of all intestine segments, jejunal goblet cell numbers, and VH-to-crypt depth ratio (VH/CD) of broilers than those of the control group (P < 0.05). Birds in the XOS + IAPS group had higher jejunal VH/CD ratio and goblet cell numbers than those from the XOS or IAPS groups (P < 0.05). In addition, there was a synergy effect between XOS and IAPS on increasing duodenal goblet cell numbers and improving ileal morphology (higher VH and VH/CD ratio) (P < 0.05). The XOS, IAPS and XOS + IAPS treatments increased the mRNA expression of zonula occludens-1 and occludin of the jejunum as compared with the control group (P < 0.05). Simultaneously, birds in the XOS + IAPS group showed lower plasma D-lactic acid concentration and higher mRNA expression of claudin-1, claudin-3, and occludin in the jejunum than those in the control or IAPS groups (P < 0.05). Moreover, there was no significant difference in growth performance, intestinal morphology, and intestinal barrier function of broilers between the AGP and XOS + IAPS groups. In conclusion, the combination of XOS and IAPS had a better potential as chlortetracycline substitute for improving the growth performance, intestinal morphology, and intestinal barrier function of broilers.
本研究旨在探讨低聚木糖（XOS）和γ-辐照黄芪多糖（IAPS）对肉鸡生长性能和肠道黏膜屏障功能的单独及联合作用。将240只1日龄罗斯308雏鸡分为5组，为期21天：对照组（基础日粮）、抗生素生长促进剂（AGP）组（基础日粮添加50 mg/kg金霉素）、XOS组（基础日粮添加100 mg/kg XOS）、IAPS组（基础日粮添加600 mg/kg IAPS）和XOS + IAPS组（基础日粮添加100 mg/kg XOS和600 mg/kg IAPS）。结果表明，XOS + IAPS组的肉鸡平均日增重（ADG）高于对照组，料重比低于对照组（P < 0.05）。与对照组相比，XOS、IAPS及XOS + IASP处理显著增加了肉鸡各肠段的绒毛高度（VH）、空肠杯状细胞数量及VH与隐窝深度比值（VH/CD）（P < 0.05）。XOS + IAPS组的空肠VH/CD比值和杯状细胞数量高于XOS组或IAPS组（P < 0.05）。此外，XOS与IAPS在增加十二指肠杯状细胞数量和改善回肠形态（更高的VH和VH/CD比值）方面具有协同效应（P < 0.05）。与对照组相比，XOS、IAPS及XOS + IAPS处理增加了空肠紧密连接蛋白-1和闭合蛋白的mRNA表达（P < 0.05）。同时，XOS + IAPS组肉鸡的血浆D-乳酸浓度低于对照组或IAPS组，空肠中闭合蛋白-1、闭合蛋白-3和闭合蛋白的mRNA表达高于对照组或IAPS组（P < 0.05）。此外，AGP组与XOS + IAPS组肉鸡的生长性能、肠道形态和肠道屏障功能无显著差异。总之，XOS与IAPS联合使用作为金霉素替代品在改善肉鸡生长性能、肠道形态和肠道屏障功能方面具有更好的潜力。
