Faculty of Pharmacy, Keio University, 1-5-30 Shibakoen, Minato, Tokyo, 105-8512, Japan.
Anal Sci. 2021 May 10;37(5):651-660. doi: 10.2116/analsci.20SCR09. Epub 2021 Jan 29.
In recent years, drug discovery and therapeutics trends have shifted from a focus on small-molecule compounds to biopharmaceuticals, genes, cell therapy, and regenerative medicine. Therefore, new approaches and technologies must be developed to respond to these changes in medical care. To achieve this, we applied a temperature-responsive separation system to purify a variety of proteins and cells. We developed a temperature-responsive chromatography technique based on a poly(N-isopropylacrylamide) (PNIPAAm)-grafted stationary phase. This method may be applied to various types of protein and cell separation applications by optimizing the properties of the modified polymers used in this system. Therefore, the developed temperature-responsive HPLC columns and temperature-responsive solid-phase extraction (TR-SPE) columns can be an effective separation tool for new therapeutic modalities such as monoclonal antibodies, nucleic acid drugs, and cells.
近年来，药物发现和治疗趋势已经从关注小分子化合物转向了生物制药、基因、细胞疗法和再生医学。因此，必须开发新的方法和技术来应对医疗保健领域的这些变化。为了实现这一目标，我们应用了一种温度响应分离系统来纯化多种蛋白质和细胞。我们开发了一种基于聚（N-异丙基丙烯酰胺）（PNIPAAm）接枝固定相的温度响应色谱技术。通过优化该系统中使用的改性聚合物的性能，可以将该方法应用于各种类型的蛋白质和细胞分离应用。因此，开发的温度响应 HPLC 柱和温度响应固相萃取（TR-SPE）柱可以成为单克隆抗体、核酸药物和细胞等新型治疗模式的有效分离工具。
