Department of Analytical Biochemistry, Meiji Pharmaceutical University, 2-522-1 Noshio, Kiyose, Tokyo, 204-8588, Japan.
Anal Sci. 2021 Aug 10;37(8):1165-1170. doi: 10.2116/analsci.20P429. Epub 2021 Jan 29.
In this study, we developed an analytical method using LC-MS/MS for the simultaneous determination of five bile acids (BAs) that have been recently reported as candidate diagnostic biomarkers for Alzheimer's disease (AD) or AD related factors in the brain. The measurement of BAs in the brains of healthy mice led to the determination of candidate diagnostic markers for AD, such as cholic acid and deoxycholic acid, and other bile acids, such as chenodeoxycholic acid noted for the ameliorating effect on the symptoms of AD. Significant positive correlations were observed between the brain and plasma concentrations of four BAs in healthy young mice. These results indicate that the BA level in the brain may be estimated by the corresponding BA level in the plasma. Thus, our study suggested that the proposed method for the analysis of the five bile acids would aid in the diagnosis of AD or in studies that use AD model mice.
在这项研究中，我们开发了一种使用 LC-MS/MS 的分析方法，用于同时测定最近报道的 5 种作为阿尔茨海默病（AD）或大脑中 AD 相关因素的候选诊断生物标志物的胆汁酸（BA）。测定健康小鼠脑中的 BA 导致了 AD 的候选诊断标志物的确定，例如胆酸和脱氧胆酸，以及其他胆汁酸，如 chenodeoxycholic acid，其对 AD 症状有改善作用。在健康年轻小鼠中，大脑和血浆中 4 种 BA 的浓度之间观察到显著的正相关。这些结果表明，大脑中 BA 的水平可以通过血浆中相应的 BA 水平来估计。因此，我们的研究表明，该 5 种胆汁酸分析方法的建立将有助于 AD 的诊断或 AD 模型小鼠的研究。
