评价选定牙龈止血剂的细胞毒性和抗菌活性-体外研究。

Assessment of cytotoxic and antimicrobial activity of selected gingival haemostatic agents - in vitro study.

机构信息

Department of Experimental Surgery and Biomaterials Research, Faculty of Dentistry, Wroclaw Medical University, Wrocław, Poland.

Department of Human Morphology and Embryology, Division of Histology and Embryology, Faculty of Medicine, Wroclaw Medical University, Wrocław, Poland.

出版信息

Acta Bioeng Biomech. 2020;22(3):185-198.

PMID:33518722

Abstract

PURPOSE

The present research aimed to determine whether and how the aluminium chloride - based materials affect the cell line of the bacterial line and fungi.

METHODS

Cytotoxicity of haemostatic astringents: Alustat (liquid), Alustat (gel), Alustat (foam), Alustin, Hemostat, Racestyptine and Traxodent containing AlCl3 was conducted on L929 cell line with the use of MTT and SRB assays. The antimicrobial activity (CFU and MIC) against C. albicans, S. mutans, L. rhamnosus was determined.

RESULTS

In the MTT results, cell viability for all agents were very low. In SRB, the lowest cytotoxicity was demonstrated for Hemostat and Alustat (foam), Traxodent and Racestyptine. Total reduction of the CFU of S. mutans was observed. Alustat (gel) and Alustat (liquid) completely inhibited the growth of C. albicans, S. mutans and L. rhamnosus.

CONCLUSIONS

The viability of L929 cells obtained in the SRB assay is more reliable than that obtained in the MTT assay, in the case of gingival haemostatic agents.

摘要

目的

本研究旨在确定基于氯化铝的材料是否以及如何影响细菌和真菌的细胞系。

方法

采用 MTT 和 SRB 法检测含 AlCl3 的止血收敛剂 Alustat（液体）、Alustat（凝胶）、Alustat（泡沫）、Alustin、Hemostat、Racestyptine 和 Traxodent 对 L929 细胞系的细胞毒性。测定其对白色念珠菌、变形链球菌、鼠李糖乳杆菌的抗菌活性（CFU 和 MIC）。

结果

在 MTT 结果中，所有药物的细胞活力都非常低。在 SRB 中，Hemostat 和 Alustat（泡沫）、Traxodent 和 Racestyptine 的细胞毒性最低。观察到变形链球菌 CFU 的完全减少。Alustat（凝胶）和 Alustat（液体）完全抑制了白色念珠菌、变形链球菌和鼠李糖乳杆菌的生长。