Nowakowska Danuta, Kulbacka Julita, Wezgowiec Joanna, Szewczyk Anna, Baczynska Dagmara, Zietek Marek, Wieckiewicz Wlodzimierz, Saczko Jolanta
Department of Prosthetic Dentistry, Wroclaw Medical University, 50-425 Wroclaw, Poland.
Department of Molecular and Cellular Biology, Wroclaw Medical University, 50-556 Wroclaw, Poland.
Materials (Basel). 2021 Apr 20;14(8):2081. doi: 10.3390/ma14082081.
Traditional chemo-mechanical retraction/displacement materials can impact the gingival margin tissues. This study was undertaken to analyze biological responses induced in human gingival fibroblasts (HGFs) upon application of injectable astringent-based agents used in the cordless retraction technique. HGFs were exposed to hemostatic agents (five gels, three pastes, and one foam) based on aluminium chloride, aluminium sulphate and ferric sulphate. Changes in cell viability and proliferation were evaluated using an MTT assay and a BrdU assay. The cytoskeleton structure organization (zyxin and F-actin) was visualized by confocal laser scanning microscopy. Oxidative stress was determined using the Griess Reagent System. The RNA expression levels of antioxidant enzymes were quantified by real-time RT-PCR. The statistical significance was evaluated using Student's t-test and one-way ANOVA with post-hoc Tukey HSD test. The evaluated agents did not downregulate fibroblast viability or proliferation. No significant cytoskeleton reorganization was observed. Only one agent (Expasyl) induced oxidative stress, demonstrated by the increased level of nitrites. Incubation with the studied agents significantly increased the RNA expression of some antioxidant enzymes (SOD1, SOD3, GPX1). However, no significant influence on the expression of SOD2 and HMOX1 was detected. The injectable forms of chemical retraction agents revealed biocompatibility with HGFs, suggesting their potential clinical usefulness in gingival margin retraction.
传统的化学机械性收缩/移位材料会影响牙龈边缘组织。本研究旨在分析在无绳收缩技术中使用的可注射收敛剂类制剂作用于人类牙龈成纤维细胞（HGFs）时所诱导的生物学反应。将HGFs暴露于基于氯化铝、硫酸铝和硫酸铁的止血剂（五种凝胶、三种糊剂和一种泡沫剂）中。使用MTT法和BrdU法评估细胞活力和增殖的变化。通过共聚焦激光扫描显微镜观察细胞骨架结构组织（桩蛋白和F-肌动蛋白）。使用格里斯试剂系统测定氧化应激。通过实时RT-PCR定量抗氧化酶的RNA表达水平。使用学生t检验和带有事后Tukey HSD检验的单因素方差分析评估统计学意义。所评估的制剂并未下调成纤维细胞的活力或增殖。未观察到明显的细胞骨架重组。只有一种制剂（Expasyl）诱导了氧化应激，表现为亚硝酸盐水平升高。与所研究的制剂孵育显著增加了一些抗氧化酶（SOD1、SOD3、GPX1）的RNA表达。然而，未检测到对SOD2和HMOX1表达的显著影响。化学收缩剂的可注射形式显示出与HGFs的生物相容性，表明它们在牙龈边缘收缩中具有潜在的临床应用价值。
