通过X射线溶液散射和单晶X射线衍射初步研究肿瘤坏死因子的结构。

Structure of tumour necrosis factor by X-ray solution scattering and preliminary studies by single crystal X-ray diffraction.

作者信息

Lewit-Bentley A, Fourme R, Kahn R, Prangé T, Vachette P, Tavernier J, Hauquier G, Niers W

机构信息

LURE, Université Paris-Sud, Orsay, France.

出版信息

J Mol Biol. 1988 Jan 20;199(2):389-92. doi: 10.1016/0022-2836(88)90323-3.

DOI:10.1016/0022-2836(88)90323-3

PMID:3351931

Abstract

The structure of tumour necrosis factor has been investigated by X-ray small-angle scattering and X-ray diffraction using synchrotron radiation. The overall radius of gyration is 25.5 A. A plausible model accounting for the scattering curves consists of an elongated trimer with an axial ratio of 3 to 4 and a maximal chord with a lower limit of 80 A. Tumour necrosis factor has been crystallized in a trigonal space group. Our results are in favour of a single trimer in the asymmetric unit. The diffraction extends to 3.5 A.

摘要

利用同步辐射通过X射线小角散射和X射线衍射对肿瘤坏死因子的结构进行了研究。其整体回转半径为25.5埃。一个能解释散射曲线的合理模型是由一个轴比为3至4的细长三聚体和一条下限为80埃的最大弦组成。肿瘤坏死因子已在一个三方空间群中结晶。我们的结果支持不对称单元中有一个单一三聚体的观点。衍射延伸至3.5埃。