Shen Sifan, Fan Zhizhi, Zhang Xun, Kong Xiangbo, Liu Fu, Zhang Zhen, Zhang Xinhua, Hu Xiumei, Zhang Sufang
Key Laboratory of Forest Protection of National Forestry and Grassland Administration, Research Institute of Forest Ecology, Environment and Protection, Chinese Academy of Forestry, Beijing, China.
College of Grassland Science and Technology, China Agricultural University, Beijing, China.
Front Genet. 2021 Jan 15;11:604757. doi: 10.3389/fgene.2020.604757. eCollection 2020.
The emerald ash borer (EAB), , is a highly destructive quarantine pest. The olfactory and visual systems of play different but critical roles at newly emerged and sexually mature stages; however, the molecular basis underlying these differences remain unclear. Consequently, based on deep transcriptome sequencing, we evaluated the expression levels of chemosensory-related proteins and opsins at the two developmental stages of . We found 15 new chemosensory-related genes in our transcriptome assembly compared with the previous genome assembly, including 6 that code for odorant-binding proteins (OBPs) and 9 for chemosensory proteins (CSPs). The expression of several chemosensory-related genes (, and ) differed markedly between newly emerged and sexually mature . We also found that the expression of and was higher in sexually mature male , which may be associated with their strong visual mate detection ability. This study forms the basis for further investigation of the chemosensory and visual system of , and these differentially expressed genes between newly emerged and sexually mature stages may serve as targets for the management of this destructive forest pest after sexual maturity.
光肩星天牛是一种极具破坏性的检疫性害虫。光肩星天牛的嗅觉和视觉系统在新羽化阶段和性成熟阶段发挥着不同但关键的作用；然而，这些差异背后的分子基础仍不清楚。因此，基于深度转录组测序，我们评估了光肩星天牛两个发育阶段化学感受相关蛋白和视蛋白的表达水平。与之前的基因组组装相比，我们在转录组组装中发现了15个新的化学感受相关基因，其中包括6个编码气味结合蛋白（OBP）的基因和9个编码化学感受蛋白（CSP）的基因。几个化学感受相关基因（……）在新羽化和性成熟的光肩星天牛之间表达差异显著。我们还发现，性成熟雄虫中……的表达较高，这可能与其较强的视觉配偶检测能力有关。本研究为进一步研究光肩星天牛的化学感受和视觉系统奠定了基础，这些在新羽化和性成熟阶段差异表达的基因可能成为该破坏性森林害虫性成熟后治理的靶点。
