Duan Jun, Quan Guoxing, Mittapalli Omprakash, Cusson Michel, Krell Peter J, Doucet Daniel
Natural Resources Canada, Great Lakes Forestry Centre, Canadian Forest Service, Sault Ste. Marie, Canada.
Department of Molecular and Cellular Biology, University of Guelph, Guelph, Canada.
Mitochondrial DNA B Resour. 2017 Feb 23;2(1):134-135. doi: 10.1080/23802359.2017.1292476.
The complete mitogenome of the Emerald Ash Borer (EAB, ) was obtained by gleaning mitochondrial sequences from whole-genome Illumina sequencing data. The circular genome has 15,942 base pairs and contains 13 protein-coding genes (PCGs), 22 transfer RNAs (tRNAs), 2 ribosomal RNAs (rRNAs) and an A-T-rich region. All PCGs begin with ATN codons. The nucleotide composition is highly asymmetric (31.65% A, 40.25% T, 17.39% G, 10.71% C), with an overall A-T content of 71.9%. Phylogenetic analysis based on insect mitogenomes indicated that EAB is closely related to other Buprestoidea species, clustering most closely with .
通过从全基因组Illumina测序数据中收集线粒体序列，获得了翡翠灰螟（EAB）的完整线粒体基因组。该环状基因组有15942个碱基对，包含13个蛋白质编码基因（PCGs）、22个转运RNA（tRNAs）、2个核糖体RNA（rRNAs）和一个富含A-T的区域。所有PCGs均以ATN密码子起始。核苷酸组成高度不对称（A占31.65%，T占40.25%，G占17.39%，C占10.71%），A-T总含量为71.9%。基于昆虫线粒体基因组的系统发育分析表明，翡翠灰螟与其他吉丁甲总科物种密切相关，与……聚类关系最为紧密。
