He Zhiping, Wu Shaozhen, Lin Ju, Booth Ashley, Rankin Gary O'Neal, Martinez Ivan, Chen Yi Charlie
The Key Laboratory for Quality Improvement of Agricultural Products of Zhejiang Province, College of Agriculture and Food Science, Zhejiang A & F University, Hangzhou 311300, China.
College of Health, Science, Technology and Mathematics, Alderson Broaddus University, Philippi, WV 26416, USA.
Appl Sci (Basel). 2020 Dec 1;10(23). doi: 10.3390/app10238615.
Ovarian cancer is the second most common gynecologic cancer with an estimated 13,940 mortalities across the United States in 2020. Natural polyphenols have been shown to double the survival time of some cancer patients due to their anticancer properties. Therefore, the effect of polyphenols extracted from Chinese hickory seed skin (CHSP) on ovarian cancer was investigated in the present study. Cell viability results showed that CHSP is more effective in inhibiting ovarian cancer cells than normal ovarian cells, with the IC50 value for inhibition of cell proliferation of Ovarian cancer cells (OVCAR-3) being 10.33 ± 0.166 μg/mL for a 24 h treatment. Flow cytometry results showed that the apoptosis rate was significantly increased to 44.21% after 24 h treatment with 20 μg/mL of CHSP. Western blot analysis showed that CHSP induced apoptosis of ovarian cancer cells through a p53-dependent intrinsic pathway. Compared with control values, levels of VEGF excreted by OVCAR-3 cancer cells were reduced to 7.87% with a 40 μg/mL CHSP treatment. Consistent with our previous reports, CHSP inhibits vascular endothelial growth factor (VEGF) secretion by regulating the HIF-1α-VEGF pathway. In addition, we also found that the inhibitory effect of CHSP on ovarian cancer is related to the up-regulation of Phosphatase and tension homolog (PTEN) and down-regulation of nuclear factor kappa-B (NF-kappa B). These findings provide some evidence of the anti-ovarian cancer properties of CHSP and support the polyphenols as potential candidates for ovarian cancer adjuvant therapy.
卵巢癌是第二常见的妇科癌症，2020年美国估计有13940人死于该病。天然多酚因其抗癌特性已被证明可使一些癌症患者的存活时间翻倍。因此，本研究调查了从山核桃籽皮中提取的多酚（CHSP）对卵巢癌的影响。细胞活力结果表明，CHSP对卵巢癌细胞的抑制作用比对正常卵巢细胞更有效，在24小时处理中，抑制卵巢癌细胞（OVCAR-3）增殖的IC50值为10.33±0.166μg/mL。流式细胞术结果表明，用20μg/mL的CHSP处理24小时后，细胞凋亡率显著增加至44.21%。蛋白质免疫印迹分析表明，CHSP通过p53依赖性内源性途径诱导卵巢癌细胞凋亡。与对照值相比，用40μg/mL的CHSP处理后，OVCAR-3癌细胞分泌的VEGF水平降至7.87%。与我们之前的报告一致，CHSP通过调节HIF-1α-VEGF途径抑制血管内皮生长因子（VEGF）的分泌。此外，我们还发现CHSP对卵巢癌的抑制作用与磷酸酶和张力蛋白同源物（PTEN）的上调和核因子κB（NF-κB）的下调有关。这些发现为CHSP的抗卵巢癌特性提供了一些证据，并支持多酚作为卵巢癌辅助治疗的潜在候选物。
