Department of Pharmacognosy and Botany, Faculty of Pharmacy, Comenius University in Bratislava, Odbojárov 10, 83232 Bratislava 3, Slovak Republic.
Department of Biochemistry, Faculty of Medicine, Masaryk University, Kamenice 5, Building A16, 62500 Brno, Czech Republic.
Molecules. 2019 Aug 12;24(16):2921. doi: 10.3390/molecules24162921.
The phenolic compounds of methanolic extracts of and were identified by liquid chromatography tandem mass spectrometry. Carnosic acid and its metabolite carnosol were the most abundant terpene phenolic compounds of , while they were completely absent in . The main terpene phenolic constituent of was 12--methylcarnosic acid and its mass/mass fragmentation pathway was explained. The detailed mechanism of carnosic acid oxidation to carnosol was suggested. The effects of extracts and/or carnosic acid, the main diterpene phenolic component of , on the proliferation and cell cycle of two melanoma cell lines (A375, Mel JuSo) and human fibroblast cell line (HFF) were investigated by MTT assay, PI-exclusion assay and flow cytometry cell cycle analysis. Extract of more efficiently than extract reduced the proliferation of the human melanoma cells. Carnosic acid showed the most significant effect. The first evidence that carnosic acid affects microtubule dynamics and arrests the cell cycle in the G2/M phase was provided. Collectively, our results demonstrate that these two species are plants of medicinal interest with perspective for further investigation. Carnosic acid could be the compound responsible for the biological activities of extracts.
通过液相色谱串联质谱法鉴定了 和 的甲醇提取物中的酚类化合物。迷迭香酸及其代谢产物迷迭香醇是 的最丰富的萜类酚类化合物，而 在 中完全不存在。 的主要萜类酚类成分是 12--甲基迷迭香酸，并解释了其质量/质量碎裂途径。提出了迷迭香酸氧化为迷迭香醇的详细机制。通过 MTT 测定法、PI 排除测定法和流式细胞术细胞周期分析，研究了 和（ ）的提取物和/或迷迭香酸（ 的主要二萜酚类成分）对两种黑素瘤细胞系（A375、Mel JuSo）和人成纤维细胞系（HFF）增殖和细胞周期的影响。与 提取物相比， 的提取物更有效地降低了人黑素瘤细胞的增殖。迷迭香酸显示出最显著的效果。提供了迷迭香酸影响微管动力学并将细胞周期阻滞在 G2/M 期的第一个证据。总的来说，我们的结果表明，这两个 物种是具有药用前景的植物，值得进一步研究。迷迭香酸可能是 提取物生物活性的化合物。
