Laboratory of Nano-design for innovative drug development, Graduate School of Pharmaceutical Sciences, Osaka University, Osaka, Japan.
Vaccine Creation Group, BIKEN Innovative Vaccine Research Alliance Laboratories, Institute for Open and Transdisciplinary Research Initiatives, Osaka University, Osaka, Japan.
Front Cell Infect Microbiol. 2021 Jan 13;10:602453. doi: 10.3389/fcimb.2020.602453. eCollection 2020.
(Mp) is a highly contagious respiratory pathogen responsible for human community-acquired pneumonia. The number of antibiotic-resistant Mp strains is increasing; therefore, to develop novel therapeutics, it is crucial to precisely understand the pathogenesis of mycoplasma pneumonia. Herein, we examined the susceptibility and response to Mp among eight inbred mouse strains. Following infection, the bacterial load in the bronchoalveolar lavage fluid (BALF) from DBA/2 mice was higher than that in the other tested strains such as BALB/c mice, which are frequently used in Mp research. In contrast, the numbers of CD45 immune cells and neutrophils in BALF were comparable between BALB/c and DBA/2 mice, with lower numbers observed in C57BL/6J and CBA/N mice than in BALB/c mice. Among the tested strains, the BALF level of interleukin 12 subunit p40 was highest in DBA/2 mice; however, significant differences in other cytokines levels were not observed between BALB/c and DBA/2 mice. After Mp infection, Mp-specific Th1 and Th17 responses were significantly enhanced in DBA/2 mice when compared with BALB/c mice. Furthermore, prior infection with Mp increased the number of neutrophils in BALF after the reinfection of DBA/2 mice through an Mp-specific CD4 T cell-dependent mechanism. Thus, DBA/2 may be an appropriate strain for evaluating Mp infection. Moreover, a comparison of responses revealed by various inbred mouse strains could be useful for elucidating the pathogenesis of Mycoplasma pneumonia.
（Mp）是一种高度传染性的呼吸道病原体，可导致人类社区获得性肺炎。对抗生素耐药的 Mp 菌株数量正在增加；因此，为了开发新的疗法，精确了解肺炎支原体的发病机制至关重要。在此，我们研究了 8 个近交系小鼠对 Mp 的敏感性和反应。感染后，DBA/2 小鼠支气管肺泡灌洗液（BALF）中的细菌负荷高于 BALB/c 等其他常用的 Mp 研究小鼠。相比之下，BALB/c 和 DBA/2 小鼠的 BALF 中 CD45 免疫细胞和中性粒细胞数量相当，而 C57BL/6J 和 CBA/N 小鼠的数量则低于 BALB/c 小鼠。在测试的菌株中，DBA/2 小鼠的 BALF 中白细胞介素 12 亚单位 p40 水平最高；但是，BALB/c 和 DBA/2 小鼠之间其他细胞因子水平没有显著差异。感染 Mp 后，与 BALB/c 小鼠相比，DBA/2 小鼠的 Mp 特异性 Th1 和 Th17 反应明显增强。此外，通过 Mp 特异性 CD4 T 细胞依赖性机制，Mp 感染可增加 DBA/2 小鼠再次感染后的 BALF 中性粒细胞数量。因此，DBA/2 可能是评估 Mp 感染的合适品系。此外，比较不同近交系小鼠的反应可能有助于阐明肺炎支原体的发病机制。
