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基于网络药理学和实验评估探索（-）-表儿茶素对卵巢早衰的作用机制。

Exploring the mechanism of (-)-Epicatechin on premature ovarian insufficiency based on network pharmacology and experimental evaluation.

机构信息

Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing, China.

School of Pharmaceutical Sciences, Tsinghua University, Beijing, China.

出版信息

Biosci Rep. 2021 Feb 26;41(2). doi: 10.1042/BSR20203955.

DOI:10.1042/BSR20203955
PMID:33521822
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7881164/
Abstract

METHODS

Relevant potential targets for EC were obtained based on Traditional Chinese Medicine System Pharmacology Database (TCMSP), a bioinformatics analysis tool for molecular mechanism of Traditional Chinese Medicine (BATMAN-TCM) and STITCH databases. The Online Mendelian Inheritance in Man (OMIM) and GeneCards databases were utilized to screen the known POI-related targets, while Cytoscape software was used for network construction and visualization. Then, the Gene Ontology (GO) and pathway enrichment analysis were carried out by the Database for Annotation, Visualization and Integrated Discovery (DAVID) database. Furthermore, KGN cells were performed to validate the predicted results in oxidative stress (OS) model, and antioxidant effect was examined.

RESULTS

A total of 70 potential common targets for EC in the treatment of POI were obtained through network pharmacology. Metabolic process, response to stimulus and antioxidant activity occupied a leading position of Gene Ontology (GO) enrichment. Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis indicated that PI3K/protein kinase B (AKT), TNF, estrogen, VEGF and MAPK signaling pathways were significantly enriched. In addition, cell experiments showed that EC exhibited antioxidant effects in an H2O2-mediated OS model in ovarian granulosa cells by regulating the expression of PI3K/AKT/nuclear factor erythroid 2-related factor 2 (Nrf2) signaling pathway and multiple downstream antioxidant enzymes.

CONCLUSION

EC could regulate multiple signaling pathways and several biological processes (BPs). EC had the ability to down-regulate elevated OS level through the PI3K/AKT/Nrf2 signaling pathway and represented a potential novel treatment for POI.

摘要

方法

基于中药系统药理学数据库（TCMSP）、中药分子机制生物信息学分析工具（BATMAN-TCM）和 STITCH 数据库，获得与 EC 相关的潜在靶点。利用在线孟德尔遗传人类数据库（OMIM）和基因卡片数据库筛选已知与 POI 相关的 POI 相关靶点，同时利用 Cytoscape 软件进行网络构建和可视化。然后，通过数据库注释、可视化和综合发现（DAVID）数据库进行基因本体论（GO）和途径富集分析。此外，在氧化应激（OS）模型中进行 KGN 细胞实验，验证预测结果，并检测抗氧化作用。

结果

通过网络药理学共获得 70 个潜在的 EC 治疗 POI 的共同靶点。代谢过程、应激反应和抗氧化活性在基因本体论（GO）富集中占据主导地位。京都基因与基因组百科全书（KEGG）分析表明，PI3K/蛋白激酶 B（AKT）、TNF、雌激素、VEGF 和 MAPK 信号通路显著富集。此外，细胞实验表明，EC 通过调节 PI3K/AKT/核因子红细胞 2 相关因子 2（Nrf2）信号通路和多种下游抗氧化酶，在卵巢颗粒细胞的 H2O2 介导的 OS 模型中表现出抗氧化作用。

结论

EC 可调节多条信号通路和多个生物学过程（BP）。EC 具有通过 PI3K/AKT/Nrf2 信号通路下调升高的 OS 水平的能力，为 POI 的潜在治疗方法提供了新的依据。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1fa7/7881164/17ce23bd85af/bsr-41-bsr20203955-g9.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1fa7/7881164/1915150f2236/bsr-41-bsr20203955-g5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1fa7/7881164/befd75f4344d/bsr-41-bsr20203955-g6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1fa7/7881164/6452f49b2db9/bsr-41-bsr20203955-g7.jpg
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1
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Biochim Biophys Acta Proteins Proteom. 2020 Jul;1868(7):140405. doi: 10.1016/j.bbapap.2020.140405. Epub 2020 Feb 28.
3
Advances in physiological functions and mechanisms of (-)-epicatechin.(-)-表儿茶素的生理功能和机制研究进展。
J Ovarian Res. 2025 Mar 22;18(1):60. doi: 10.1186/s13048-025-01639-w.
4
Role of nuclear factor erythroid 2-related factor 2 (Nrf2) in female and male fertility.核因子红细胞2相关因子2（Nrf2）在雌性和雄性生育中的作用。
Heliyon. 2024 Apr 16;10(9):e29752. doi: 10.1016/j.heliyon.2024.e29752. eCollection 2024 May 15.
5
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6
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Front Nutr. 2022 May 20;9:875765. doi: 10.3389/fnut.2022.875765. eCollection 2022.
Crit Rev Food Sci Nutr. 2021;61(2):211-233. doi: 10.1080/10408398.2020.1723057. Epub 2020 Feb 23.
4
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7
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8
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Oxid Med Cell Longev. 2019 Dec 2;2019:7591840. doi: 10.1155/2019/7591840. eCollection 2019.
9
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