The Basic Research of Classic Recipe Application Innovation Research Group, College of Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing 100029, China.
J Tradit Chin Med. 2021 Feb;41(1):167-180. doi: 10.19852/j.cnki.jtcm.20201208.001.
To investigate the targets and mechanisms of action of Qingkailing injection (，QKL) in the treatment of cholestatic hepatitis.
A network pharmacology method was implemented using drug and disease databases to target QKL and cholestasis hepatitis, respectively. The functional protein association network STRING database was used to construct a protein-protein interaction network using R language and the Bioconductor toolkit. The org.Hs.eg.db and clusterProfiler packages were used for gene ontology and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis, which explored biological functions and pathways of potential targets. Targets were then visualized using Cytoscape 3.6.0 software.
We screened 121 compounds in QKL and identified 112 targets for the treatment of cholestatic hepatitis. QKL played a role in the treatment of cholestatic hepatitis through 305 biology process terms, 15 cellular component and 29 molecular function terms. The mechanism of QKL action was mainly related to tumor necrosis factor, mitogen-activated protein kinase, and PI3K-Akt signaling pathways.
The treatment of cholestatic hepatitis by QKL involved multiple targets, biological functions, and signaling pathways that are closely associated with the disease.
研究清开灵注射液（QKL）治疗胆汁淤积性肝炎的作用靶点及作用机制。
分别利用药物和疾病数据库，采用网络药理学方法对 QKL 和胆汁淤积性肝炎的靶点进行研究。利用 R 语言和 Bioconductor 工具包中的 STRING 数据库构建蛋白质-蛋白质相互作用网络。使用 org.Hs.eg.db 和 clusterProfiler 软件包进行基因本体和京都基因与基因组百科全书通路富集分析，以探讨潜在靶点的生物学功能和通路。然后使用 Cytoscape 3.6.0 软件可视化靶点。
我们从 QKL 中筛选出 121 种化合物，鉴定出 112 个治疗胆汁淤积性肝炎的靶点。QKL 通过 305 个生物学过程术语、15 个细胞成分和 29 个分子功能术语发挥治疗胆汁淤积性肝炎的作用。QKL 的作用机制主要与肿瘤坏死因子、丝裂原活化蛋白激酶和 PI3K-Akt 信号通路有关。
QKL 治疗胆汁淤积性肝炎涉及多个靶点、生物学功能和与疾病密切相关的信号通路。
