Univ Lille, CNRS, INRAE, Centrale Lille, UMR 8207 - UMET - Unité Matériaux et Transformations, Lille, France.
Univ Lyon, Université Claude Bernard Lyon 1, CNRS, LAGEPP UMR 5007, Villeurbanne, France.
Biofouling. 2021 Jan;37(1):49-60. doi: 10.1080/08927014.2021.1873958. Epub 2021 Jan 31.
Dodecyltrimethylammonium chloride (DTAC) was trapped into maltodextrins/pectin spray dried microcapsules to improve its activity against enteritidis and biofilms. Two different microcapsules were prepared: uncomplexed DTAC-microcapsules (UDM), containing DTAC and maltodextrins; and complexed DTAC-microcapsules (CDM) containing DTAC complexed with pectin and maltodextrins. The minimum inhibitory concentrations (MIC) of both free and microencapsulated DTAC were investigated against Enteritidis and . The MICs of DTAC were significantly lower when encapsulated. CDM treatment resulted in a 2 and 3.2 log reduction in and Enteritidis biofilm culturable biomass, respectively. Microencapsulation reduced the cytotoxicity of DTAC by up to 32-fold. Free DTAC and CDM targeted the cell membrane resulting in the leakage of the intracellular molecules and subsequent cell death. The development of DTAC microcapsules reduced the amount of DTAC required to maintain the high standards of cleanliness and hygiene required in the food processing industries.
十二烷基三甲基氯化铵（DTAC）被包埋在麦芽糊精/果胶喷雾干燥微胶囊中，以提高其对肠沙门氏菌和生物膜的活性。制备了两种不同的微胶囊：未络合的 DTAC-微胶囊（UDM），含有 DTAC 和麦芽糊精；和络合的 DTAC-微胶囊（CDM），含有与果胶和麦芽糊精络合的 DTAC。研究了游离和微囊化 DTAC 对肠沙门氏菌和 的最小抑菌浓度（MIC）。包封后，DTAC 的 MIC 值显著降低。CDM 处理分别使 和 肠沙门氏菌生物膜可培养生物量减少了 2 和 3.2 个对数。微囊化将 DTAC 的细胞毒性降低了多达 32 倍。游离 DTAC 和 CDM 靶向细胞膜，导致细胞内分子泄漏，随后细胞死亡。DTAC 微胶囊的开发减少了在食品加工行业中维持高标准清洁和卫生所需的 DTAC 用量。
