Departments of Biology, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.
Departments of Pharmacology, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.
Plant Signal Behav. 2021 Apr 3;16(4):1878685. doi: 10.1080/15592324.2021.1878685. Epub 2021 Jan 31.
A role for the heterotrimeric G protein complex in the induction of a transient burst of reactive oxygen species (ROS) by the Microbial-Associated Molecular Pattern, flg22, a 22-amino acid peptide derived from bacterial flagella, is well established. However, the evidence for a negative or positive role for one component of the Arabidopsis G protein complex, namely, Regulator of G Signaling 1 (AtRGS1) leads to opposing conclusions. We show that the reason for this difference is due to the isolate of Col-0 ecotype used as the wildtype control in flg22-induced ROS and our data further support the idea that AtRGS1 is a negative regulator of the flg22-induced ROS response. Whole-genome genotyping led to the identification and validation of polymorphism in five genes between two Col-0 isolates that are candidates for the different ROS response relative to the null mutant.
由细菌鞭毛衍生的 22 个氨基酸肽 flg22 可诱导活性氧簇（ROS）短暂爆发，异三聚体 G 蛋白复合物在此过程中发挥作用，这一观点已得到广泛认可。然而，对于拟南芥 G 蛋白复合物的一个组成部分，即 G 蛋白信号调节因子 1（AtRGS1），其在其中所起的作用是抑制还是促进作用，目前的证据却得出了截然相反的结论。我们发现，导致这一差异的原因在于用作 flg22 诱导 ROS 的野生型对照的 Col-0 生态型分离株，并且我们的数据进一步支持了这样一种观点，即 AtRGS1 是 flg22 诱导 ROS 反应的负调节剂。全基因组基因分型导致在两个 Col-0 分离株之间鉴定和验证了五个候选基因的多态性，这些基因相对于 null 突变体在 ROS 反应方面存在差异。
