T-2毒素、HT-2毒素和T-2四醇在生物体液中的稳定性。

Stability of T-2, HT-2, and T-2 tetraol in biological fluids.

作者信息

Pace J G, Matson C F

机构信息

U.S. Army Medical Research Institute of Infectious Diseases, Frederick, Maryland 21701-5011.

出版信息

J Anal Toxicol. 1988 Jan-Feb;12(1):48-50. doi: 10.1093/jat/12.1.48.

DOI:10.1093/jat/12.1.48

PMID:3352243

Abstract

The stabilities of tritium-labeled T-2, HT-2, and T-2 tetraol were studied in blood and urine at -70 degrees, 4 degrees, and 23 degrees C for 6 months in the presence of EDTA or NaF. Samples were counted with a radiochromatographic scanner and results indicated the stability of T-2 tetraol greater than T-2 greater than HT-2. Toxins were most stable when stored at -70 degrees C, in the presence of NaF, and in urine (pH 6). They were less stable in saline (control, pH 7) and least stable in blood (pH 8). These results suggest that urine and T-2 tetraol are the biological fluid and metabolite of choice for diagnostic purposes.

摘要

在存在乙二胺四乙酸（EDTA）或氟化钠（NaF）的情况下，研究了氚标记的T-2、HT-2和T-2四醇在-70℃、4℃和23℃下于血液和尿液中的稳定性，为期6个月。用放射性色谱扫描仪对样品进行计数，结果表明T-2四醇的稳定性大于T-2大于HT-2。毒素在-70℃、存在NaF且在尿液（pH 6）中储存时最稳定。它们在生理盐水（对照，pH 7）中稳定性较差，在血液（pH 8）中最不稳定。这些结果表明，尿液和T-2四醇是用于诊断目的的首选生物体液和代谢物。