Rood H D, Swanson S P, Buck W B
J Chromatogr. 1986 Jun 13;378(2):375-83. doi: 10.1016/s0378-4347(00)80733-1.
A rapid and easy procedure to screen for trichothecenes in plasma and urine is presented. The toxins are extracted using a Clin-Elut column, hydrolyzed to their corresponding parent alcohols and cleaned up with a silica cartridge followed by derivatization for gas chromatographic analysis. The detection of any of the parent alcohols in plasma or urine would indicate an exposure to trichothecenes. Recoveries in urine are between 78 and 119% at levels of 50-1000 ng/ml and recoveries in plasma are between 80 and 116% at levels of 50-500 ng/ml. The limit of detection is better than 25 ppb.
本文介绍了一种快速简便的血浆和尿液中镰刀菌毒素筛查方法。毒素采用Clin-Elut柱提取，水解为相应的母体醇，并用硅胶柱净化，然后进行衍生化用于气相色谱分析。血浆或尿液中任何一种母体醇的检测都表明接触过镰刀菌毒素。尿液中50-1000 ng/ml水平的回收率在78%至119%之间，血浆中50-500 ng/ml水平的回收率在80%至116%之间。检测限优于25 ppb。
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