Jiangsu Key Laboratory for Microbes and Functional Genomics, Jiangsu Engineering and Technology Research Centre for Industrialization of Microbial Resources, College of Life Sciences, Nanjing Normal University, Nanjing, 210023, China.
Institute of Environment, Resource, Soil and Fertilizer, Zhejiang Academy of Agricultural Sciences, Hangzhou, 310021, China.
Environ Sci Pollut Res Int. 2021 Jun;28(22):27998-28013. doi: 10.1007/s11356-021-12569-7. Epub 2021 Feb 1.
Composting has become the most important way to recycle medicinal herbal residues (MHRs). The traditional composting method, adding a microbial agent at one time, has been greatly limited due to its low composting efficiency, mutual influence of microbial agents, and unstable compost products. This study was conducted to assess the effect of multi-phase inoculation on the lignocellulose degradation, enzyme activities, and fungal community during MHRs composting. The results showed that multi-phase inoculation treatment had the highest thermophilic temperature (68.2 °C) and germination index (102.68%), significantly improved available phosphorus content, humic acid, and humic substances concentration, accelerated the degradation of cellulose and lignin, and increased the activities of cellulase in the mature phase, xylanase, manganese peroxidase, and utilization of phenolic compounds. Furthermore, the non-metric multi-dimensional scaling showed that the composting process and inoculation significantly influenced fungal community composition. In multi-phase inoculation treatment, Thermomyces in mesophilic, thermophilic, and mature phase, unclassified_Sordariales, and Coprinopsis in mature phase were the dominant genus that might be the main functional groups to degrade lignocellulose and improve the MHRs composting process.
堆肥已成为回收药用植物残渣（MHRs）的最重要方法。由于传统的一次添加微生物菌剂的堆肥方法存在堆肥效率低、微生物菌剂相互影响以及堆肥产品不稳定等问题，其应用受到了极大的限制。本研究旨在评估多阶段接种对 MHRs 堆肥过程中木质纤维素降解、酶活性和真菌群落的影响。结果表明，多阶段接种处理具有最高的高温温度（68.2°C）和发芽指数（102.68%），显著提高了有效磷含量、腐殖酸和腐殖质浓度，加速了纤维素和木质素的降解，并提高了成熟阶段纤维素酶、木聚糖酶、锰过氧化物酶和酚类化合物利用率的活性。此外，非度量多维标度显示，堆肥过程和接种显著影响了真菌群落组成。在多阶段接种处理中，中温、高温和成熟阶段的嗜热毛壳菌、未分类的 Sordariales 和成熟阶段的毛头鬼伞是主要的属，可能是降解木质纤维素和改善 MHRs 堆肥过程的主要功能群。
