College of Resources and Environmental Science, Yunnan Agricultural University, Kunming 650201, China.
Organic Recycling Institute (Suzhou) of China Agricultural University, Wuzhong District, Suzhou 215128, China; College of Resources and Environmental Science, Beijing Key Laboratory of Biodiversity and Organic Farming, China Agricultural University, 100193 Beijing, China.
Bioresour Technol. 2023 Sep;384:129291. doi: 10.1016/j.biortech.2023.129291. Epub 2023 Jun 7.
This study aimed to explore the effect of regulating pH and Phanerochaete chrysosporium inoculation at the cooling stage of composting on the lignocellulose degradation, humification process and related precursors as well as fungal community for secondary fermentation. Results showed that composting with P. chrysosporium inoculation and pH regulation (T4) had 58% cellulose decomposition, 73% lignin degradation and improved enzyme activities for lignin decomposition. There was 81.98% increase of humic substance content and more transformation of polyphenols and amino acids in T4 compared to control. Inoculating P. chrysosporium affected the fungal community diversity, and regulating pH helped to increase the colonization of P. chrysosporium. Network analysis showed that the network complexity and synergy between microorganisms was improved in T4. Correlation and Random Forest analysis suggested that enriched Phanerochaete and Thermomyces in the mature stage of T4 were key taxa for lignocellulose degradation, and humic acid formation by accumulating precursors.
本研究旨在探索在堆肥冷却阶段调节 pH 值和接种黄孢原毛平革菌对二次发酵中木质纤维素降解、腐殖化过程及相关前体物和真菌群落的影响。结果表明，接种黄孢原毛平革菌并调节 pH 值（T4）的堆肥处理具有 58%的纤维素分解率、73%的木质素降解率和提高的木质素分解酶活性。与对照组相比，T4 中的腐殖质含量增加了 81.98%，多酚和氨基酸的转化更多。接种黄孢原毛平革菌影响了真菌群落的多样性，而调节 pH 值有助于增加黄孢原毛平革菌的定殖。网络分析表明，T4 中微生物之间的网络复杂性和协同作用得到了提高。相关性和随机森林分析表明，T4 成熟阶段富集的 Phanerochaete 和 Thermomyces 是木质纤维素降解和积累前体物形成腐殖酸的关键分类群。
