Perianez-Rodriguez Juan, Rodriguez Marcos, Marconi Marco, Bustillo-Avendaño Estefano, Wachsman Guy, Sanchez-Corrionero Alvaro, De Gernier Hugues, Cabrera Javier, Perez-Garcia Pablo, Gude Inmaculada, Saez Angela, Serrano-Ron Laura, Beeckman Tom, Benfey Philip N, Rodríguez-Patón Alfonso, Del Pozo Juan Carlos, Wabnik Krzysztof, Moreno-Risueno Miguel A
Centro de Biotecnología y Genómica de Plantas (Universidad Politécnica de Madrid-Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria). Campus de Montegancedo, Pozuelo de Alarcón, 28223 Madrid, Spain.
Departamento de Inteligencia Artificial, ETSIINF, Universidad Politécnica de Madrid, 28040 Madrid, Spain.
Sci Adv. 2021 Jan 1;7(1). doi: 10.1126/sciadv.abd4722. Print 2021 Jan.
In , the root clock regulates the spacing of lateral organs along the primary root through oscillating gene expression. The core molecular mechanism that drives the root clock periodicity and how it is modified by exogenous cues such as auxin and gravity remain unknown. We identified the key elements of the oscillator (AUXIN RESPONSE FACTOR 7, its auxin-sensitive inhibitor IAA18/POTENT, and auxin) that form a negative regulatory loop circuit in the oscillation zone. Through multilevel computer modeling fitted to experimental data, we explain how gene expression oscillations coordinate with cell division and growth to create the periodic pattern of organ spacing. Furthermore, gravistimulation experiments based on the model predictions show that external auxin stimuli can lead to entrainment of the root clock. Our work demonstrates the mechanism underlying a robust biological clock and how it can respond to external stimuli.
在根中，根时钟通过振荡基因表达调节侧生器官沿主根的间距。驱动根时钟周期性的核心分子机制以及它如何被生长素和重力等外源信号修饰仍不清楚。我们鉴定出振荡器的关键元件（生长素响应因子7、其生长素敏感抑制剂IAA18/强效因子和生长素），它们在振荡区形成一个负调控回路。通过与实验数据拟合的多级计算机建模，我们解释了基因表达振荡如何与细胞分裂和生长协调，以产生器官间距的周期性模式。此外，基于模型预测的重力刺激实验表明，外部生长素刺激可导致根时钟的同步。我们的工作揭示了一个强大生物钟的潜在机制以及它如何对外源刺激做出反应。
