School of Biology,M. V. Lomonosov Moscow State University, Moscow, 119991,Russian Federation.
N. D. Zelinsky Institute of Organic Chemistry,Russian Academy of Sciences, Leninsky Prospect, 47, Moscow, 119991,Russian Federation.
Carbohydr Res. 2021 Feb;500:108247. doi: 10.1016/j.carres.2021.108247. Epub 2021 Jan 23.
The cell wall of Clavibacter michiganensis subsp. michiganensis VKM Ас-1403 (family Microbacteriaceae, class Actinobacteria) contains two polysaccharides. The first one is neutral (1 → 6) linked galactofuranan in which every second galactofuranose residue in the main chain substituted at position 3 by side trisaccharide, β-D-GlcpNAc-(1 → 3)-α-L-Rhap-(1 → 2)-α-D-Fucp-(1 →. The second polymer is pyruvylated galactomannan with the repeating unit, →3)-α-D-Galp-(1 → 3)-α-D-[4,6-S-Pyr]-Manp-(1 → 3)-α-D-Manp-(1 →. The cell wall glycopolymer structures were established by chemical and NMR spectroscopic methods. The obtained results provide new data on the cell wall composition of plant pathogenic species of the genus Clavibacter and can promote understanding the molecular mechanisms involved in colonization and infection of plants.
类枝孢菌亚种密歇根变种（微杆菌科，放线菌纲）的细胞壁含有两种多糖。第一种是中性（1→6）连接的半乳呋喃聚糖，主链中的每个半乳呋喃糖残基在第 3 位被侧三糖取代，β-D-GlcpNAc-（1→3）-α-L-Rhap-（1→2）-α-D-Fucp-（1→。第二种聚合物是丙酮酸化半乳甘露聚糖，其重复单元为→3）-α-D-Galp-（1→3）-α-D-[4,6-S-Pyr]-Manp-（1→3）-α-D-Manp-（1→。细胞壁糖聚合物结构通过化学和 NMR 光谱方法确定。所得结果提供了关于植物病原性类枝孢属种细胞壁组成的新数据，并有助于理解定植和感染植物的分子机制。
