CAS Key Laboratory of Magnetic Resonance in Biological Systems, State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics, National Center for Magnetic Resonance in Wuhan, Wuhan Institute of Physics and Mathematics, Innovation Academy for Precision Measurement Science and Technology, Chinese Academy of Sciences (CAS), Wuhan 430071, China.
CAS Key Laboratory of Magnetic Resonance in Biological Systems, State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics, National Center for Magnetic Resonance in Wuhan, Wuhan Institute of Physics and Mathematics, Innovation Academy for Precision Measurement Science and Technology, Chinese Academy of Sciences (CAS), Wuhan 430071, China; University of Chinese Academy of Sciences, Beijing 100049, China.
Ecotoxicol Environ Saf. 2021 Apr 1;212:111989. doi: 10.1016/j.ecoenv.2021.111989. Epub 2021 Jan 30.
Drinking water exposure to microcystin-leucine-arginine (MC-LR), the most widely occurring cyanotoxins, poses a highly potential risk for human health. However, the health risk of MC-LR exposure at current guideline value in drinking water has not yet entirely evaluated. In the current study, we used H NMR-based metabolomics combined with targeted metabolic profiling by GC/LC-MS to explore the toxic effects of MC-LR exposure at environmentally relevant concentrations via drinking water in rats. The results revealed that multiple biological consequences of MC-LR exposure on host metabolism in rats. Both relatively low and high doses of MC-LR used here induced hepatic lipogenesis and inflammation. While only relatively high dose MC-LR (10 μg/L) in drinking water caused more metabolic disorders including inhibition of gluconeogenesis and promotion of β-oxidation of fatty acid. Although the dose of 1.0 μg/L MC-LR is extremely low for rats, alterations of metabolic profiles were unexpectedly found in rat liver and serum, alarming potential health risk of MC-LR at the WHO guideline level.
饮用水中暴露于微囊藻毒素-亮氨酸-精氨酸（MC-LR），这是最广泛存在的蓝藻毒素，对人类健康构成了高度潜在的风险。然而，饮用水中现行指导值下 MC-LR 暴露的健康风险尚未得到全面评估。在本研究中，我们使用基于 1H NMR 的代谢组学结合 GC/LC-MS 的靶向代谢分析，来探究通过饮用水暴露于环境相关浓度的 MC-LR 对大鼠的毒性作用。结果揭示了 MC-LR 暴露对大鼠宿主代谢的多种生物学后果。这里使用的低剂量和高剂量 MC-LR 均诱导了肝内脂质生成和炎症。而只有低剂量 MC-LR（10μg/L）在饮用水中引起了更多的代谢紊乱，包括抑制糖异生和促进脂肪酸的β氧化。尽管对于大鼠来说，1.0μg/L MC-LR 的剂量极低，但在大鼠肝脏和血清中却意外地发现了代谢谱的改变，这令人警惕 MC-LR 在世界卫生组织指导值水平下的潜在健康风险。
