Effect of continuous wave, quasi-continuous wave and pulsed laser radiation on functional characteristics of fish spermatozoa.

For the first time, using sturgeon sperm as a model system, sensitive to optical radiation, the comparative studies of biological effect of continuous wave, quasi-continuous wave, nano- and picosecond laser radiation under conditions with equal average irradiance (3 mW/cm) and wavelength (532 nm) have been carried out. Analyzing the parameters of spermatozoa motion it has been shown that, depending on the energy dose and mode of laser operation, the radiation may have both stimulatory and inhibitory effect on the velocity of motion and spermatozoa motility duration as well as on sustaining of functional characteristics of cold-stored sperm. The possibility of increasing the fertilization rate due to use of the sperm preliminary treated with laser radiation is demonstrated. For the first time, the possibility of enhancement of biological effect going from continuous wave to quasi-continuous wave laser radiation at equal irradiance and wavelength has experimentally been proven. It is shown that the difference in biological effect of continuous wave, quasi-continuous wave, nano- and picosecond laser radiation is due to amplitude (peak) values of intensity. Using fluorescence analysis and luminol-dependent chemiluminescence assay, evidence for the participation of endogenous flavins and metal-free porphyrins in sensitized ROS formation (singlet oxygen, hydrogen peroxide, and hydroxyl radicals) in sturgeon sperm was obtained. Mechanisms of photochemical and photothermal reactions explaining the difference in efficacy of action of laser radiation in above modes are discussed.