Yeung C H, De Geyter C, De Geyter M, Nieschlag E
Institute of Reproductive Medicine, University, Münster, Germany.
J Assist Reprod Genet. 1996 Jul;13(6):495-500. doi: 10.1007/BF02066531.
Reactive oxygen species (ROS) including H2O2 produced by spermatozoa have been suggested, on one hand, to be associated with idiopathic male infertility and, on the other hand, to stimulate certain sperm function leading to fertilization. The influence of ROS on fertilization was investigated in 75 IVF patients by correlating fertilization rates with the production of ROS and the H2O2-scavenging activity of swim-up spermatozoa prepared in parallel with the IVF samples.
Low rates of ROS production by the swim-up sperm was detected by the luminol-dependent chemiluminescence assay. They were not correlated with fertilization rates. The hydrogen peroxide scavenging capacity of these spermatozoa, measured as the removal of exogenous H2O2 assayed spectrophotometrically, decreased stepwise in groups of patients achieving higher fertilization rates, suggesting a positive effect of this ROS on fertilization. An alternative explanation of this correlation is plausible in view of the association of both high scavenging activities and poor fertilization rates with poor sperm morphology.
ROS produced by spermatozoa selected by swim-up plays no negative, if not a positive, role in fertilization.
一方面，已有人提出包括精子产生的过氧化氢（H₂O₂）在内的活性氧（ROS）与特发性男性不育有关；另一方面，ROS可刺激某些精子功能从而导致受精。通过将受精率与ROS的产生以及与体外受精（IVF）样本平行制备的上浮精子的H₂O₂清除活性相关联，研究了ROS对75例IVF患者受精的影响。
通过鲁米诺依赖性化学发光测定法检测到上浮精子产生的ROS水平较低。它们与受精率无关。这些精子的过氧化氢清除能力，通过分光光度法测定的外源H₂O₂的去除量来衡量，在受精率较高的患者组中逐步降低，这表明这种ROS对受精有积极作用。鉴于高清除活性和低受精率都与精子形态差有关，这种相关性的另一种解释也是合理的。
上浮法选择的精子产生的ROS在受精过程中即使没有积极作用，也没有消极作用。
