Wang Weizhen, Fang Yuan, Imran Muhammad, Hu Zhihong, Zhang Sicong, Huang Zhongqiao, Liu Xili
Department of Plant Pathology, College of Plant Protection, China Agricultural University, Beijing 100193, China.
State Key Laboratory of Crop Stress Biology for Arid Areas, Northwest A&F University, Yangling 712110, China.
Microorganisms. 2021 Jan 28;9(2):266. doi: 10.3390/microorganisms9020266.
is a destructive necrotrophic pathogen that can infect many plant species. The control of gray mold mainly relies on the application of fungicides, and the fungicide fludioxonil is widely used in China. However, the field fungicide resistance of to this compound is largely unknown. In this study, isolates were collected from different districts of Shanghai province in 2015-2017, and their sensitivity to fludioxonil was determined. A total of 65 out of 187 field isolates (34.76%) were found to be resistant to fludioxonil, with 36 (19.25%) showing high resistance and 29 (15.51%) showing moderate resistance. Most of these resistant isolates also showed resistance to iprodione, and some developed resistance to fungicides of other modes of action. gene expression, an indicator of MDR1 and MDR1h phenotypes, was not dramatically increased in the tested resistant isolates. Biological characteristics and osmotic sensitivity investigations showed that the fitness of resistant isolates was lower than that of sensitive ones. To investigate the molecular resistance mechanisms of to fludioxonil, the Bos1 amino acid sequences were compared between resistant and sensitive isolates. Resistant isolates revealed either no amino acid variations or the mutations I365S, I365N, Q369P/N373S, and N373S.
是一种具有破坏性的坏死营养型病原体，可感染多种植物物种。灰霉病的防治主要依赖于杀菌剂的应用，氟啶胺在中国被广泛使用。然而，其对该化合物的田间杀菌剂抗性情况 largely unknown。在本研究中，于2015 - 2017年从上海不同地区收集了 isolates，测定了它们对氟啶胺的敏感性。在187个田间分离株中，共有65个（34.76%）被发现对氟啶胺具有抗性，其中36个（19.25%）表现出高抗性，29个（15.51%）表现出中等抗性。这些抗性分离株大多也对异菌脲具有抗性，一些还对其他作用方式的杀菌剂产生了抗性。作为MDR1和MDR1h表型指标的 基因表达，在测试的抗性分离株中并未显著增加。生物学特性和渗透敏感性研究表明，抗性分离株的适应性低于敏感分离株。为了研究 对氟啶胺的分子抗性机制，比较了抗性和敏感分离株之间的Bos1氨基酸序列。抗性分离株要么没有氨基酸变异，要么存在I365S、I365N、Q369P/N373S和N373S突变。
原文中“largely unknown”和“isolates”未明确具体内容，翻译时保留原文形式。
