Biochemistry Department, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.
Student Research Committee, Shiraz University of Medical Sciences, Shiraz, Iran.
Gynecol Endocrinol. 2021 Aug;37(8):764-768. doi: 10.1080/09513590.2021.1879784. Epub 2021 Feb 1.
Estrogen (E2) deficiency has been related to uterine metabolic dysfunction, which could be accompanied by infertility in the reproductive ages. Despite having adverse effects, estrogen replacement therapy is considered the fundamental treatment strategy for this problem. The current study sought to determine the palliative effects of quercetin (Q) and vitamin E (Vit.E) on some of the uterine's metabolism-related factors in ovariectomized (OVX) rats and compare them with the effects of estrogen.
Sixty-four rats were divided into eight groups. OVX animals were treated with Q (15 mg/kg/day), Vit.E (60 mg/kg/day), E2 (10 µg/kg/day), and Q (7.5 mg/kg/day) + Vit.E (30 mg/kg/day) for 10 weeks. Glucose and adiponectin were measured using glucose oxidase and ELISA, respectively. Furthermore, the present study investigated the alterations in the expression of AdipoR1, nesfatin1, and GluT4 genes.
Antioxidants suppress the weight gain of OVX animals. Also, Q, Vit.E, and E2 cause a significant decline in glucose and adiponectin levels (-value < .05). Finally, the expression of AdipoR1, nesfatin1, and GLUT4 genes was significantly increased in treated OVX rats' uterus.
The present findings suggest that the administration of Q and Vit.E could demonstrate promising characteristics in a similar approach with estradiol and thus be considered as alternatives for estrogen replacement therapy.
雌激素（E2）缺乏与子宫代谢功能障碍有关，这可能伴随着生殖期的不孕。尽管雌激素替代疗法有不良影响，但它被认为是治疗这种问题的基本策略。本研究旨在确定槲皮素（Q）和维生素 E（Vit.E）对去卵巢（OVX）大鼠子宫一些与代谢相关的因素的缓解作用，并将其与雌激素的作用进行比较。
将 64 只大鼠分为 8 组。OVX 动物用 Q（15mg/kg/天）、Vit.E（60mg/kg/天）、E2（10μg/kg/天）和 Q（7.5mg/kg/天）+Vit.E（30mg/kg/天）治疗 10 周。葡萄糖和脂联素分别用葡萄糖氧化酶和 ELISA 测定。此外，本研究还研究了 AdipoR1、nesfatin1 和 GluT4 基因表达的变化。
抗氧化剂抑制 OVX 动物的体重增加。此外，Q、Vit.E 和 E2 可显著降低血糖和脂联素水平（-值<.05）。最后，治疗后的 OVX 大鼠子宫中 AdipoR1、nesfatin1 和 GLUT4 基因的表达显著增加。
本研究结果表明，槲皮素和维生素 E 的给药可能在与雌二醇类似的方法中表现出有希望的特性，因此可以被认为是雌激素替代疗法的替代品。
