Guo Kai, Qi Di, Huang Bo
Department of Thoracic Surgery, The First Affiliated Hospital of Jinzhou Medical University, Renming Street #5-2, Guta District, Jinzhou City, Liaoning Province, 121000, People's Republic of China.
Cancer Cell Int. 2021 Feb 1;21(1):84. doi: 10.1186/s12935-021-01772-8.
Non-small cell lung cancer (NSCLC) is the most common tumor with severe morbidity and high mortality. Long non-coding RNAs (lncRNAs) as crucial regulators participate in multiple cancer progressions. However, the role of lncRNA MEG8 in the development of NSCLC remains unclear. Here, we aimed to investigate the effect of lncRNA MEG8 on the progression of NSCLC and the underlying mechanism.
Cell proliferation was analyzed by EdU assays. The impacts of lncRNA MEG8, miR-15a-5p, and miR-15b-5p on cell invasion and migration of NSCLC were assessed by transwell assay. The luciferase reporter gene assay was performed using the Dual-luciferase Reporter Assay System. The effect of lncRNA MEG8, miR-15a-5p, and miR-15b-5p on tumor growth was evaluated in nude mice of Balb/c in vivo.
We revealed that the expression levels of MEG8 were elevated in the NSCLC patient tissues compared to that in adjacent normal tissues. The expression of MEG8 was negatively relative to that of miR-15a-5p and miR-15b-5p in the NSCLC patient tissues. The expression of MEG8 was upregulated, while miR-15a-5p and miR-15b-5p were downregulated in NSCLC cell lines. The depletion of MEG8 inhibited NSCLC cell proliferation, migration, and invasion in vitro. MEG8 contributed to NSCLC progression by targeting miR-15a-5p/miR-15b-5p in vitro. LncRNA MEG8 contributes to tumor growth of NSCLC via the miR-15a/b-5p/PSAT1 axis in vivo. Thus, we concluded that lncRNA MEG8 promotes NSCLC progression by modulating the miR-15a/b-5p/PSAT1 axis.
Our findings demonstrated that lncRNA MEG8 plays a critical role in NSCLC development. LncRNA MEG8, miR-15a-5p, miR-15b-5p, and PSAT1 may serve as potential targets for NSCLC therapy.
非小细胞肺癌（NSCLC）是最常见的肿瘤，发病率高且死亡率高。长链非编码RNA（lncRNAs）作为关键调节因子参与多种癌症进展。然而，lncRNA MEG8在NSCLC发生发展中的作用仍不清楚。在此，我们旨在研究lncRNA MEG8对NSCLC进展的影响及其潜在机制。
通过EdU检测分析细胞增殖。采用Transwell检测评估lncRNA MEG8、miR-15a-5p和miR-15b-5p对NSCLC细胞侵袭和迁移的影响。使用双荧光素酶报告基因检测系统进行荧光素酶报告基因检测。在Balb/c裸鼠体内评估lncRNA MEG8、miR-15a-5p和miR-15b-5p对肿瘤生长的影响。
我们发现，与相邻正常组织相比，NSCLC患者组织中MEG8的表达水平升高。在NSCLC患者组织中，MEG8的表达与miR-15a-5p和miR-15b-5p的表达呈负相关。在NSCLC细胞系中，MEG8的表达上调，而miR-15a-5p和miR-15b-5p的表达下调。MEG8的缺失在体外抑制NSCLC细胞增殖、迁移和侵袭。在体外，MEG8通过靶向miR-15a-5p/miR-15b-5p促进NSCLC进展。lncRNA MEG8在体内通过miR-15a/b-5p/PSAT1轴促进NSCLC肿瘤生长。因此，我们得出结论，lncRNA MEG8通过调节miR-15a/b-5p/PSAT1轴促进NSCLC进展。
我们的研究结果表明，lncRNA MEG8在NSCLC发生发展中起关键作用。lncRNA MEG8、miR-15a-5p、miR-15b-5p和PSAT1可能作为NSCLC治疗的潜在靶点。
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