对六种伊朗蝎子的线粒体标记物细胞色素氧化酶亚基I（COI）、12S核糖体DNA（rDNA）和16S核糖体DNA（rDNA）的分子分析
Molecular analysis of the mitochondrial markers COI, 12S rDNA and 16S rDNA for six species of Iranian scorpions.
作者信息
Soltan-Alinejad Parisa, Rafinejad Javad, Dabiri Farrokh, Onorati Piero, Terenius Olle, Chavshin Ali Reza
机构信息
Social Determinants of Health, Research Center, Urmia University of Medical Sciences, Urmia, Iran.
Department of Medical Entomology and Vector Control, School of Public Health, Urmia University of Medical Sciences, Urmia, Iran.
出版信息
BMC Res Notes. 2021 Feb 1;14(1):40. doi: 10.1186/s13104-021-05449-3.
OBJECTIVES
Annually, 1.2 million humans are stung by scorpions and severely affected by their venom. Some of the scorpion species of medical importance have a similar morphology to species with low toxicity. To establish diagnostic tools for surveying scorpions, the current study was conducted to generate three mitochondrial markers, Cytochrome Oxidase I (COI gene), 12S rDNA and 16S rDNA for six species of medically important Iranian scorpions: Androctonus crassicauda, Hottentotta saulcyi, Mesobuthus caucasicus, M. eupeus, Odontobuthus doriae, and Scorpio maurus.
RESULTS
Phylogenetic analyses of the obtained sequences corroborated the morphological identification. For the first time, 12S rDNA sequences are reported from Androctonus crassicauda, Hottentotta saulcyi, Mesobuthus caucasicus and M. eupeus and also the 16S rDNA sequence from Hottentotta saulcyi. We conclude that the mitochondrial markers are useful for species determination among these medically important species of scorpions.
目的
每年有120万人被蝎子蜇伤并深受其毒液影响。一些具有医学重要性的蝎子物种与低毒性物种形态相似。为建立用于蝎子调查的诊断工具，本研究针对六种具有医学重要性的伊朗蝎子——粗壮肥尾蝎、索氏肥尾蝎、高加索杀牛蝎、真杀牛蝎、多氏刺尾蝎和东疆蝎，生成了三个线粒体标记，即细胞色素氧化酶I（COI基因）、12S rDNA和16S rDNA。
结果
对所得序列的系统发育分析证实了形态学鉴定结果。首次报道了粗壮肥尾蝎、索氏肥尾蝎、高加索杀牛蝎和真杀牛蝎的12S rDNA序列，以及索氏肥尾蝎的16S rDNA序列。我们得出结论，线粒体标记对于这些具有医学重要性的蝎子物种的鉴定很有用。
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