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黑肥尾蝎不同种群线粒体基因的分子调查

Molecular Survey of Mitochondrial Genes in Different Populations of the Black Fat-Tailed Scorpion, .

作者信息

Ghavami Mohammad Bagher, Alibabaei Zohreh, Ghavami Fatemeh

机构信息

Department of Medical Entomology and Vector Control, School of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran.

Department of Electronics, Faculty of Electrical and Computer Engineering, Tarbiat Modares University, Tehran, Iran.

出版信息

J Arthropod Borne Dis. 2022 Jun 30;16(2):84-96. doi: 10.18502/jad.v16i2.11799. eCollection 2022 Jun.

DOI:10.18502/jad.v16i2.11799
PMID:37038503
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10082414/
Abstract

BACKGROUND

is the most medically relevant scorpion and understanding its genetic forms is essential for improvement of anti-venom sera, and risk management of scorpionism. Present study was designed to identify the variations of mitochondrial genes in different populations of .

METHODS

Adults of were collected from Zanjan Province during 2016-2017. Genomic DNA of samples was extracted and fragments of mitochondrial 16S, COI and ND1 genes were amplified and some of the amplicons were sequenced. Haplotype of samples were identified by multiple alignment of sequences, then phylogenetic trees of haplotypes were constructed.

RESULTS

Fragments of 352bp, 618bp and 680bp were amplified from 16S, COI and ND1 genes respectively. Nucleotide sequence in COI fragments was conserved, however, five haplotypes with some specific polymorphic sites were detected in 16S and ND1 fragments. Haplotype I was dominant and found in all areas. Other haplotypes were rare and limited to specific regions. Analysis of the phylogenetic trees inferred from 16S and COI genes, confirmed a strong positive correlation between geographic and genetic distance.

CONCLUSION

Mitochondrial COI, 16S and ND1 genes were detected suitable for identifying the population structure. Five genotypes were found using 16S and ND1 genes. To prepare and improve the anti-venoms quality, additional studies are necessary to identify the toxin electrophoretic profile and geographical/ecological niche models of these genotypes in future.

摘要

背景

[蝎子种类名称]是医学上最具相关性的蝎子，了解其基因形式对于改进抗毒血清以及蝎蜇伤的风险管理至关重要。本研究旨在鉴定[蝎子种类名称]不同种群中线粒体基因的变异情况。

方法

2016 - 2017年期间从赞詹省采集[蝎子种类名称]成虫。提取样本的基因组DNA，扩增线粒体16S、COI和ND1基因片段，并对部分扩增产物进行测序。通过序列多重比对鉴定样本的单倍型，然后构建单倍型的系统发育树。

结果

分别从16S、COI和ND1基因扩增出352bp、618bp和680bp的片段。COI片段中的核苷酸序列保守，然而，在16S和ND1片段中检测到五个具有一些特定多态性位点的单倍型。单倍型I占主导地位，在所有区域均有发现。其他单倍型罕见且局限于特定区域。对由16S和COI基因推断出的系统发育树的分析证实了地理距离和遗传距离之间存在很强的正相关。

结论

检测到线粒体COI、16S和ND1基因适用于鉴定种群结构。使用16S和ND1基因发现了五种基因型。为了制备和提高抗毒液质量，未来需要进行更多研究以鉴定这些基因型的毒素电泳图谱和地理/生态位模型。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9ad1/10082414/b970ab6e3dee/JAD-16-84-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9ad1/10082414/e759f29c6ae1/JAD-16-84-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9ad1/10082414/e6ebafb72ca0/JAD-16-84-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9ad1/10082414/a0861c505e9a/JAD-16-84-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9ad1/10082414/94ac6d6e46ec/JAD-16-84-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9ad1/10082414/37fed077d2fd/JAD-16-84-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9ad1/10082414/431ebc042089/JAD-16-84-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9ad1/10082414/b970ab6e3dee/JAD-16-84-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9ad1/10082414/e759f29c6ae1/JAD-16-84-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9ad1/10082414/e6ebafb72ca0/JAD-16-84-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9ad1/10082414/a0861c505e9a/JAD-16-84-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9ad1/10082414/94ac6d6e46ec/JAD-16-84-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9ad1/10082414/37fed077d2fd/JAD-16-84-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9ad1/10082414/431ebc042089/JAD-16-84-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9ad1/10082414/b970ab6e3dee/JAD-16-84-g007.jpg

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7
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Iran J Pharm Res. 2014 Summer;13(3):743-56.
8
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9
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