Cold Spring Harb Protoc. 2021 Feb 1;2021(2):2021/2/pdb.prot102137. doi: 10.1101/pdb.prot102137.
Many expression vectors make use of the operon. In general, the operator () is located downstream from the promoter of the target gene, so that binding of the repressor blocks transcription initiation until lactose or the isopropyl-β-d-thiogalactopyranoside (IPTG) analog is added. The protocol given here is intended for use with IPTG-inducible vectors. l-Arabinose-inducible systems derived from the operon offer an alternative to expression systems based on the operon; guidance for their use is also provided.
许多表达载体利用操纵子。一般来说，操纵基因（operator）位于目标基因启动子的下游，因此，当阻遏物结合时，转录起始被阻止，直到添加乳糖或异丙基-β-D-硫代半乳糖苷（IPTG）类似物。这里给出的方案旨在与 IPTG 诱导型载体一起使用。来自操纵子的 l-阿拉伯糖诱导系统为基于操纵子的表达系统提供了替代方案；也提供了它们使用的指南。
