Taylor E M, Geyer J R, Milstein J M, Shaw C M, Geraci J P, Wootton P, Hubbard B A, Bleyer W A
Department of Medicine, Jennifer Sacco International Research Laboratories, Children's Hospital, Seattle, WA.
NCI Monogr. 1988(6):59-64.
This report describes a laboratory model that permits study of the radiochemotherapy interactions in the CNS. Rats are stereotaxically implanted with a cerebroventricular cannula attached to an osmotic minipump, which slowly infuses a chemotherapeutic agent into CSF for up to 14 days. The cervical cord is irradiated, and forelimb paralysis develops 4-6 months later at an effective dose for paresis in 50% of the animals; the doses with radiotherapy alone are 2,125 cGy for a single fraction and 2,950 cGy for split fractions. Investigations with the model indicate that mature CNS tissue is not sensitized to either single-fraction or split-dose irradiation with either simultaneous or post-radiation exposure to high concentrations of methotrexate.
本报告描述了一种实验室模型，该模型可用于研究中枢神经系统中的放化疗相互作用。将大鼠通过立体定位植入一根连接到渗透微型泵的脑室插管，该微型泵可将化疗药物缓慢注入脑脊液中，持续长达14天。对颈髓进行照射，4至6个月后，50%的动物在前肢出现瘫痪，产生麻痹的有效剂量为单剂量2125 cGy和分次剂量2950 cGy。对该模型的研究表明，成熟的中枢神经系统组织对单剂量或分次剂量照射均不敏感，无论在照射期间还是照射后同时暴露于高浓度的甲氨蝶呤。
