Stratil P, Rejthar A, Lang B A, Kocent A, Pacovský Z, Matoska J
Research Institute of Clinical and Experimental Oncology, Brno, Czechoslovakia.
Neoplasma. 1988;35(1):69-76.
In 116 breast cancer patients, the levels of carcinoembryonic antigen (CEA) were determined before operation in the serum using RIA, and after operation in sections of breast tumor tissue using the immunohistological PAP technique. CEA circulating in the serum was found in 49 patients (42%). Elevated values (over 10 micrograms/l) were found in only 12 patients (10%). In histological specimens CEA positivity was found in 94 tumors (81%), however, in a majority of them the number of positive cells per section was low (1-10%). A comparison of positive and negative findings both in the serum and in the tumor specimens of individual patients showed that both serum and tumor sections were CEA positive in 40 patients (35%) and both localizations were CEA negative in 13 patients (12%). Although most patients had positive histological sections but negative sera (46%). Only 7% of patients had negative sections and positive sera. In 41 patients CEA could be examined both qualitatively (immunohistologically), and quantitatively in the cytosol of the same homogenized tumor. Of them, 30 patients (72%) had in the cytosol a CEA concentration exceeding 5 micrograms/g proteins, in 11 of the 41 patients (28%) no CEA was found. Immunohistological examination of CEA in this group gave positive results in 35 out of the 41 patients (85%), and only 6 tumors (15%) were completely negative. CEA was shown to be present in each histological type of the tumors studied, invasive ductal tumors being slightly more frequent and more positive than the lobular ones. No relation was observed to the structure of the tumors, nor to the degree of their differentiation. Thus, the examination of CEA levels can hardly contribute to the improvement of histological classification.
对116例乳腺癌患者，术前采用放射免疫分析法（RIA）测定血清中癌胚抗原（CEA）水平，术后采用免疫组织化学PAP技术检测乳腺肿瘤组织切片中的CEA水平。49例患者（42%）血清中检测到循环CEA。仅12例患者（10%）CEA值升高（超过10微克/升）。在组织学标本中，94个肿瘤（81%）检测到CEA阳性，但其中大多数切片中阳性细胞数量较少（1%-10%）。对个体患者血清和肿瘤标本的阳性和阴性结果进行比较显示，40例患者（35%）血清和肿瘤切片均为CEA阳性，13例患者（12%）两个部位均为CEA阴性。尽管大多数患者组织学切片阳性但血清阴性（46%）。仅7%的患者切片阴性但血清阳性。41例患者的CEA既能进行定性（免疫组织化学）检测，也能对同一匀浆肿瘤的细胞溶质进行定量检测。其中，30例患者（72%）细胞溶质中CEA浓度超过5微克/克蛋白，41例患者中的11例（28%）未检测到CEA。该组中对CEA进行免疫组织化学检测，41例患者中有35例（85%）结果为阳性，仅6个肿瘤（15%）完全阴性。研究表明，在所有研究的肿瘤组织学类型中均存在CEA，浸润性导管癌比小叶癌略更常见且阳性率更高。未观察到与肿瘤结构及其分化程度的关系。因此，检测CEA水平对改善组织学分类几乎没有帮助。
