Guangxi Medical University, Department First School of Clinical Medicine.
Department of Spine and Osteopathic Surgery, the First Affiliated Hospital of Guangxi Medical University, Nanning, P. R. China.
Medicine (Baltimore). 2021 Jan 29;100(4):e24484. doi: 10.1097/MD.0000000000024484.
Ewing sarcoma (ES), the second most prevalent bone malignant tumor has no widely known prognostic biomarker. Earlier studies have suggested that chaperonin containing TCP1 complex 6A (CCT6A), which encodes a molecular protein chaperone, is involved in the pathogenesis of many cancers. However, there are no known reports providing clear evidence of its role in ES pathogenesis.
We performed a bioinformatic analysis of 32 ES specimens from the GSE17618 dataset concentrating on the differences in gene expression, OS, event-free survival (EFS) in the different subgroups. Immunohistochemical studies were also performed to identify the expression levels of selected genes in ES and immediate paracancerous tissues.
After 3 screenings, CCT6A was identified to be highly correlated with ES prognosis. Our survival analysis revealed a low overall survival (OS) for high CCT6A expression (P-value = .024). Our Cox regression analysis identified CCT6A expression, lEFS, and age were strongly associated with prognosis of ES. Our multivariate Cox regression analysis shows that CCT6A (P-value = .015), age (P-value = .026), and EFS (P-value = .002) were independent poor prognostic biomarkers. Our immunohistochemical analysis showed that the expression levels of CCT6A were significantly higher in ES tissues compared to the paracancerous tissues.
From the results of our study, we identified the expression levels of CCT6A to be strongly associated with prognosis of ES. Thus, the expression levels of the CCT6A gene could serve as a biomarker for the prediction of ES prognosis.
尤因肉瘤（ES）是第二大常见的骨恶性肿瘤，目前尚无广泛认可的预后生物标志物。早期研究表明，热休克蛋白 10 家族成员 1A（HSP10A），编码分子伴侣蛋白，参与许多癌症的发病机制。然而，目前尚无已知的报道提供明确的证据表明其在 ES 发病机制中的作用。
我们对来自 GSE17618 数据集的 32 个 ES 标本进行了生物信息学分析，重点关注不同亚组之间基因表达、OS 和无事件生存（EFS）的差异。还进行了免疫组织化学研究，以确定选定基因在 ES 和紧邻癌旁组织中的表达水平。
经过 3 次筛选，CCT6A 被确定与 ES 预后高度相关。我们的生存分析显示，高 CCT6A 表达的总生存期（OS）较低（P 值=0.024）。我们的 Cox 回归分析确定 CCT6A 表达、LEFS 和年龄与 ES 的预后密切相关。我们的多变量 Cox 回归分析显示，CCT6A（P 值=0.015）、年龄（P 值=0.026）和 EFS（P 值=0.002）是独立的不良预后生物标志物。我们的免疫组织化学分析显示，CCT6A 在 ES 组织中的表达水平明显高于癌旁组织。
从我们的研究结果来看，我们确定了 CCT6A 的表达水平与 ES 的预后密切相关。因此，CCT6A 基因的表达水平可以作为预测 ES 预后的生物标志物。
