Pradelli Jennifer, Tuccia Fabiola, Giordani Giorgia, Vanin Stefano
School of Applied Sciences, University of Huddersfield, Queensgate, Huddersfield HD1 3DH, UK.
Dipartimento di Farmacia e Biotecnologie (FABIT), Alma Mater Studiorum Università di Bologna, 40126 Bologna, Italy.
Insects. 2021 Jan 26;12(2):104. doi: 10.3390/insects12020104.
Diptera puparia may represent both in forensic and archaeo-funerary contexts the majority of the entomological evidence useful to reconstruct the peri and post-mortem events. Puparia identification is quite difficult due to the lack of identification keys and descriptions. In addition, external substances accumulated during the puparia permanence in the environment make the visualization of the few diagnostic characters difficult, resulting in a wrong identification. Six different techniques based on physical and chemical treatments have been tested for the removal of external substances from puparia to make identification at species level feasible. Furthermore, the effects of these methods on successful molecular analyses have also been tested as molecular identification is becoming an important tool to complement morphological identifications. The results of this study indicate that cleaning via warm water/soap, the sonication and treatment with a sodium hydroxide solution are the best methods to achieve a good quality of the samples.
在法医和考古丧葬背景下，双翅目蛹可能代表了大部分有助于重建生前和死后事件的昆虫学证据。由于缺乏鉴定检索表和描述，蛹的鉴定相当困难。此外，蛹在环境中留存期间积累的外部物质使得少数诊断特征难以显现，从而导致错误鉴定。已经测试了六种基于物理和化学处理的不同技术，用于去除蛹上的外部物质，以使物种水平的鉴定可行。此外，由于分子鉴定正成为补充形态学鉴定的重要工具，这些方法对成功进行分子分析的影响也已得到测试。本研究结果表明，用温水/肥皂清洗、超声处理和用氢氧化钠溶液处理是获得高质量样本的最佳方法。
