Immunology Research Center (IRC), Institute of Immunology and Infectious Disease, Iran University of Medical Sciences, Shahid Hemmat Highway 14496, Tehran, Iran.
Department of Immunology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
BMC Cardiovasc Disord. 2021 Feb 2;21(1):68. doi: 10.1186/s12872-021-01892-z.
Coronary artery disease (CAD) is characterized by narrowing/ blockade of coronary arteries that is mainly caused by atherosclerotic plaques. Considering the involvement of platelet abnormalities, such as defective aggregation and adhesion, in the cardiovascular-related disorders, genetic variations in human platelet alloantigens (HPA) have been implicated in the CAD susceptibility. Herein, we intended to determine the association of HPA-1 to -6, -9, and -15 biallelic polymorphisms with CAD in an Iranian population.
In this retrospective case-control study, 200 CAD subjects and 100 matched healthy individuals were enrolled. DNA samples were isolated from peripheral blood samples and genotyping of HPA polymorphisms was accomplished using polymerase chain reaction-sequence-specific primers.
The alleles and genotypes of studied HPA polymorphisms were equally distributed among cases and controls and therefore no statistically significant differences were detected. Univariate analysis identified no association of combined haplotypes with CAD risk. However, multivariate analysis showed a positive association of the HPA1b/2a/3b haplotype with CAD after adjustment for some covariates (including BMI, TG, LDL, FBS and blood pressure) that conferred a CAD susceptibility haplotype (P = 0.015; OR = 2.792; 95% CI 1.45-8.59).
Although alleles, genotypes, and haplotypes of HPA polymorphisms were not associated with CAD risk, HPA1b/2a/3b haplotype was found to be a dependent disease risk haplotype in Iranian population after correcting for confounding factors.
冠心病（CAD）的特征是冠状动脉狭窄/阻塞，主要由动脉粥样硬化斑块引起。考虑到血小板异常（如聚集和黏附缺陷）在心血管相关疾病中的参与，人类血小板同种抗原（HPA）的遗传变异与 CAD 易感性有关。在此，我们旨在确定 HPA-1 至-6、-9 和-15 双等位基因多态性与伊朗人群 CAD 的关联。
在这项回顾性病例对照研究中，纳入了 200 名 CAD 患者和 100 名匹配的健康个体。从外周血样本中提取 DNA 样本，并使用聚合酶链反应-序列特异性引物对 HPA 多态性进行基因分型。
研究 HPA 多态性的等位基因和基因型在病例和对照组中均匀分布，因此未检测到统计学上的显著差异。单变量分析未发现组合单倍型与 CAD 风险之间存在关联。然而，多变量分析显示，在调整一些协变量（包括 BMI、TG、LDL、FBS 和血压）后，HPA1b/2a/3b 单倍型与 CAD 呈正相关，该单倍型为 CAD 易感单倍型（P=0.015；OR=2.792；95%CI 1.45-8.59）。
尽管 HPA 多态性的等位基因、基因型和单倍型与 CAD 风险无关，但在校正混杂因素后，发现 HPA1b/2a/3b 单倍型是伊朗人群中独立的疾病风险单倍型。
