Department of Molecular Medicine, Cornell University, Ithaca, NY 14853, USA.
Department of Chemistry and Chemical Biology, Cornell University, Ithaca, NY 14853, USA.
STAR Protoc. 2021 Jan 22;2(1):100295. doi: 10.1016/j.xpro.2021.100295. eCollection 2021 Mar 19.
Cells produce two broad classes of extracellular vesicles (EVs), exosomes and microvesicles (MVs). Exosomes are 30-150 nm vesicles derived from multivesicular bodies, while MVs are 200-1,000 nm vesicles that pinch off from plasma membranes. Reliable isolation of EVs is crucial to understand their biochemical and functional properties. Here, we describe a protocol to isolate and characterize EVs from conditioned medium from mammalian cell lines. This protocol has been optimized for adherent cells but can also be adapted for suspension cells. For complete details on the use and execution of this protocol, please refer to Latifkar et al. (2019).
细胞产生两类广泛的细胞外囊泡（EVs），即外泌体和微泡（MVs）。外泌体是源自多泡体的 30-150nm 囊泡，而 MVs 是从质膜出芽形成的 200-1000nm 囊泡。可靠地分离 EVs 对于了解其生化和功能特性至关重要。在这里，我们描述了一种从哺乳动物细胞系的条件培养基中分离和表征 EVs 的方案。该方案已针对贴壁细胞进行了优化，但也可以适应悬浮细胞。有关此方案的使用和执行的完整详细信息，请参阅 Latifkar 等人。（2019 年）。
