School of Pharmacy, Shanghai Jiao Tong University, 800 Dongchuan Road, 200240, Shanghai, China.
Engineering Research Center of Cell & Therapeuti c Antibody, Ministry of Education, and School of Pharmacy, Shanghai Jiao Tong University, Shanghai 200240, China.
Biomater Sci. 2024 Feb 27;12(5):1131-1150. doi: 10.1039/d3bm01980b.
Extracellular vesicles (EVs) are vesicles with lipid bilayer structures shed from the plasma membrane of cells. Microvesicles (MVs) are a subset of EVs containing proteins, lipids, nucleic acids, and other metabolites. MVs can be produced under specific cell stimulation conditions and isolated by modern separation technology. Due to their tumor homing and large volume, tumor cell-derived microvesicles (TMVs) have attracted interest recently and become excellent delivery carriers for therapeutic vaccines, imaging agents or antitumor drugs. However, preparing sufficient and high-purity TMVs and conducting clinical transformation has become a challenge in this field. In this review, the recent research achievements in the generation, isolation, characterization, modification, and application of TMVs in cancer therapy are reviewed, and the challenges facing therapeutic applications are also highlighted.
细胞外囊泡 (EVs) 是由细胞质膜脱落的具有双层脂膜结构的囊泡。微囊泡 (MVs) 是 EVs 的一个亚类，其中包含蛋白质、脂质、核酸和其他代谢物。MVs 可以在特定的细胞刺激条件下产生，并通过现代分离技术进行分离。由于其具有肿瘤归巢和较大的体积，肿瘤细胞来源的微囊泡 (TMVs) 最近引起了人们的兴趣，并成为治疗性疫苗、成像剂或抗肿瘤药物的优秀递送载体。然而，制备足够数量和高纯度的 TMVs 并进行临床转化已成为该领域的一个挑战。本文综述了 TMVs 在癌症治疗中的生成、分离、表征、修饰和应用方面的最新研究成果，并强调了其在治疗应用上面临的挑战。
