A facile synthesis of long-wavelength emission nitrogen-doped carbon dots for intracellular pH variation and hypochlorite sensing.

Intracellular pH and hypochlorite (ClO) concentration play an important role in life activities, so there is an urgent need to develop a valid strategy to monitor pH and ClO in biological systems with high sensitivity and specificity. In this study, we report long-wavelength emission nitrogen-doped carbon dots (N-CDs) and their potential applications in intracellular pH variation, ClO sensing and cell imaging. The N-CDs were prepared via a facile one-pot hydrothermal method of neutral red (NR) and glutamine (Gln). N-CDs exhibited a pH-sensitive response in the range of 4.0-9.0 and a good linear relationship in the range of 5.6-7.4, which indicated that N-CDs are an ideal agent for monitoring pH fluctuations in living cells. In addition, ClO was capable of reducing the photoluminescence of N-CDs based on static quenching. The linear range is 1.5-112.5 μM and 112.5-187.5 μM, and the LOD is 0.27 μM. Besides, the as-fabricated N-CDs have been smoothly achieved to monitor pH and ClO in PC-12 living cells due to their great biocompatibility and lower cytotoxicity, demonstrating their promising applications in the biomedical field. Compared with other CD-based methods, the as-proposed N-CDs have a longer fluorescence emission, which makes them potentially valuable in biological systems. The results pave a way towards the construction of long-wavelength carbon-based nanomaterials for fluorescence sensing and cell imaging.