离子液体分散液液微萃取法同时测定生物样品中卡马西平和拉莫三嗪的含量。
Ionic Liquid-Based Dispersive Liquidâ€"Liquid Microextraction for the Simultaneous Determination of Carbamazepine and Lamotrigine in Biological Samples.
出版信息
Acta Chim Slov. 2020 Sep;67(3):748-756.
This paper describes a new approach for the determination of carbamazepine and lamotrigine in biological samples by ionic liquid dispersive liquid-phase microextraction prior to high-performance liquid chromatography with ultraviolet detection. The effects of different ionic liquids (ILs) on the extraction efficiency of carbamazepine and lamotrigine were investigated. The highest extraction efficiencies of carbamazepine and lamotrigine were obtained using 30 ?L of 1-me-thyl-3-octylimidazolium hexafluorophosphate [C8MIM][PF6]. Several factors affecting the microextraction efficiency, such as the type and volume of extracting solvent, type and volume of disperser solvent, salt concentration, and pH of the sample solution have been optimized. The calibration plots were linear in the range of 0.1-20 mg L-1 for carbamazepine and 0.3-40 mg L-1 for lamotrigine with detection limits of 0.04 mg L-1 for carbamazepine and 0.07 mg L-1 for lamotrig-ine in plasma samples. The results confirm the suitability of the presented method as a sensitive method for the analysis of the target analytes in urine and plasma samples.
本文描述了一种新的方法，用于通过离子液体分散液相微萃取（IL-DLLME）在高效液相色谱-紫外检测（HPLC-UV）之前，对生物样品中的卡马西平和拉莫三嗪进行测定。考察了不同离子液体（ILs）对卡马西平和拉莫三嗪萃取效率的影响。使用 30 μL 的 1-甲基-3-辛基咪唑六氟磷酸盐 [C8MIM][PF6]，可获得卡马西平和拉莫三嗪的最高萃取效率。优化了影响微萃取效率的几个因素，如萃取溶剂的类型和体积、分散剂溶剂的类型和体积、盐浓度和样品溶液的 pH 值。卡马西平在 0.1-20 mg L-1 范围内、拉莫三嗪在 0.3-40 mg L-1 范围内呈线性，卡马西平在血浆样品中的检测限为 0.04 mg L-1，拉莫三嗪为 0.07 mg L-1。结果证实，该方法适用于尿液和血浆样品中目标分析物的灵敏分析。
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