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含有 miR-451a 的外泌体参与了脑缺血预处理对脑缺血再灌注损伤的保护作用。

Exosomes containing miR-451a is involved in the protective effect of cerebral ischemic preconditioning against cerebral ischemia and reperfusion injury.

机构信息

Stroke Center, Changhai Hospital, Shanghai, China.

Department of neurosurgery, Changhai Hospital, Shanghai, China.

出版信息

CNS Neurosci Ther. 2021 May;27(5):564-576. doi: 10.1111/cns.13612. Epub 2021 Feb 3.

DOI:10.1111/cns.13612
PMID:33533575
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8025619/
Abstract

AIM

To study the role of exosomes in the protective effect of cerebral ischemic preconditioning (cerebral-IPC) against cerebral I/R injury.

METHOD

Mouse models of cerebral-IPC and MCAO/R were established as described previously, and their behavioral, pathological, and proteomic changes were analyzed. Neuro-2a subjected to OGD/R were treated with exosomes isolated from the plasma of sham-operated and cerebral-IPC mice. The differentially expressed miRNAs between exosomes derived from sham-operated (S-exosomes) and preconditioned (IPC-exosomes) mice were identified through miRNA array, and their targets were identified through database search. The control and OGD/R cells were treated with the IPC-exosomes, miRNA mimic or target protein inhibitor, and their viability, oxidative, stress and apoptosis rates were measured. The activated pathways were identified by analyzing the levels of relevant proteins.

RESULTS

Cerebral-IPC mitigated the cerebral injury following ischemia and reperfusion, and increased the number of plasma exosomes. IPC-exosomes increased the survival of Neuro-2a cells after OGD/R. The miR-451a targeting Rac1 was upregulated in the IPC-exosomes relative to S-exosomes. The miR-451a mimic and the Rac1 inhibitor NSC23766 reversed OGD/R-mediated activation of Rac1 and its downstream pathways.

CONCLUSION

Cerebral-IPC ameliorated cerebral I/R injury by inducing the release of exosomes containing miR-451a.

摘要

目的

研究细胞外囊泡在脑缺血预处理（脑-IPC）对脑缺血/再灌注损伤的保护作用中的作用。

方法

如前所述，建立了脑-IPC 和 MCAO/R 小鼠模型，分析了其行为、病理和蛋白质组学变化。用来自假手术和脑 IPC 小鼠血浆的外泌体处理神经-2a 细胞，以进行氧葡萄糖剥夺/复氧（OGD/R）。通过 miRNA 阵列鉴定了来自假手术（S-外泌体）和预处理（IPC-外泌体）小鼠的外泌体中差异表达的 miRNA，通过数据库搜索鉴定了它们的靶标。用 IPC-外泌体、miRNA 模拟物或靶蛋白抑制剂处理对照和 OGD/R 细胞，测量其存活率、氧化应激和凋亡率。通过分析相关蛋白的水平来鉴定激活的途径。

结果

脑 IPC 减轻了缺血再灌注后的脑损伤，并增加了血浆外泌体的数量。IPC-外泌体增加了神经-2a 细胞在 OGD/R 后的存活率。与 S-外泌体相比，IPC-外泌体中 Rac1 的靶标 miR-451a 上调。miR-451a 模拟物和 Rac1 抑制剂 NSC23766 逆转了 OGD/R 介导的 Rac1 及其下游途径的激活。

结论

脑 IPC 通过诱导含有 miR-451a 的外泌体释放来改善脑 I/R 损伤。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/522a/8025619/996f367c0144/CNS-27-564-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/522a/8025619/5e4a9bb6a239/CNS-27-564-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/522a/8025619/124e0b997b83/CNS-27-564-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/522a/8025619/1d6bd8001a73/CNS-27-564-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/522a/8025619/1eb512cfab3c/CNS-27-564-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/522a/8025619/25f3b975d9bf/CNS-27-564-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/522a/8025619/996f367c0144/CNS-27-564-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/522a/8025619/5e4a9bb6a239/CNS-27-564-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/522a/8025619/124e0b997b83/CNS-27-564-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/522a/8025619/1d6bd8001a73/CNS-27-564-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/522a/8025619/1eb512cfab3c/CNS-27-564-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/522a/8025619/25f3b975d9bf/CNS-27-564-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/522a/8025619/996f367c0144/CNS-27-564-g007.jpg

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3
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4
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5
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6
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7
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