Garza Luis, Bitar Ryan, O'Donnell Barrett, Parker Matthew, Ortiz Carlos, Hyman Charles, Walker John, Song Ho-Young, Lopera Jorge
Long School of Medicine, University of Texas Health Science Center, 7703 Floyd Curl Drive San Antonio, San Antonio, TX, 78229, USA.
Department of Radiology, University of Texas Health Science Center, San Antonio, TX, USA.
CVIR Endovasc. 2021 Feb 3;4(1):20. doi: 10.1186/s42155-021-00210-0.
To develop an ex- vivo perfusion flow model using a bovine kidney for future testing of embolic agents in an inexpensive and easy way.
Six bovine adult kidneys were used for this study. Kidneys were cannulated and perfused via a roller pump. Three embolic agents, coils, Gelfoam, and a glue mixture of Histoacryl + Lipiodol, were deployed by targeting three secondary segmental arteries per kidney via a 5Fr catheter under fluoroscopic guidance. Cannulation time, success rate of segmental artery selection and embolic agent deployment, total operational time, and fluoroscopy dose were recorded.
Average kidney weight was 0.752 +/- 0.094 kg. All six bovine kidneys were successfully cannulated in 21.6 min +/- 3.0 min. Deployment of coils and glue was achieved in every case (12/12); however, Gelfoam injection was not successful in one instance (5/6, 83%). Coil deployment demonstrated no embolic effect while Gelfoam and glue injections demonstrated decreased distal contrast filling post-embolization. Mean dose area product was 12.9 ± 1.8 Gy·cm2, fluoroscopy time was 10 ± 4 min and operational time was 27 ± 8 min.
We describe the creation of an ex vivo bovine kidney flow model for the preclinical evaluation of different embolic materials. The flow model can be modified to provide extensive bench testing and it is a promising tool for hands -on training in basic and advanced embolization techniques .
开发一种使用牛肾的体外灌注血流模型，以便以低成本且简便的方式对栓塞剂进行未来测试。
本研究使用了6个成年牛肾。通过滚轴泵对肾脏进行插管和灌注。在荧光透视引导下，通过一根5Fr导管针对每个肾脏的三条二级节段动脉部署三种栓塞剂，即弹簧圈、明胶海绵以及一种由组织黏合剂+碘油组成的胶水混合物。记录插管时间、节段动脉选择和栓塞剂部署的成功率、总操作时间以及透视剂量。
牛肾平均重量为0.752±0.094千克。所有6个牛肾均在21.6分钟±3.0分钟内成功插管。每种栓塞剂（弹簧圈和胶水）的部署均成功（12/12）；然而，明胶海绵注射有1例未成功（5/6，83%）。弹簧圈部署未显示栓塞效果，而明胶海绵和胶水注射显示栓塞后远端造影剂充盈减少。平均剂量面积积为12.9±1.8 Gy·cm²，透视时间为10±4分钟，操作时间为27±8分钟。
我们描述了一种用于不同栓塞材料临床前评估的体外牛肾血流模型的创建。该血流模型可进行修改以提供广泛的实验台测试，并且是基础和高级栓塞技术实践培训的一个有前景的工具。
