South Texas Radiology Group, University of Texas Health Science Center at San Antonio, San Antonio, Texas, USA.
J Vasc Interv Radiol. 2011 Oct;22(10):1478-83. doi: 10.1016/j.jvir.2011.06.013. Epub 2011 Jul 28.
To describe an in vitro blood-perfused bovine liver model for the testing of radiofrequency (RF) ablation devices and compare the performance of a specific RF ablation device in the model relative to three other biologic models.
Fresh bovine livers were used to create three in vitro models: blood-perfused, Krebs-Henseleit (KH) solution-perfused, and nonperfused. The perfused models were connected to a heart-lung machine via the portal vein and perfused with heparinized autologous blood or KH solution under physiologic conditions. Six swine were used as in vivo liver models. A cluster electrode and RF ablation system was operated in impedance mode for 12 minutes in all models. Ablated livers were sectioned, with long- and short-axis measurements of the ablations obtained, and statistical analysis was performed.
A total of 39, 23, 17, and 12 ablations were performed in 14, 6, 5, and 6 blood-perfused bovine livers, KH solution-perfused bovine livers, nonperfused bovine livers, and in vivo porcine livers, respectively. On cut specimens, the average diameters of ablation zones were 4.00 cm (95% CI, 3.88-4.13) in blood-perfused livers, 4.34 cm (95% CI, 4.14-4.50) in KH solution-perfused livers, 4.67 cm (95% CI, 4.50-4.83) in nonperfused livers, and 3.56 cm (95% CI, 3.26-3.83) in in vivo porcine livers. In all models, the ablation zone diameters were normally distributed.
In the in vitro blood-perfused bovine liver model, the size of ablations produced by an RF ablation device are closer in size to those seen in porcine liver in vivo compared with the lesions produced in KH solution-perfused or nonperfused bovine liver.
描述一种用于测试射频（RF）消融设备的体外血液灌注牛肝模型，并比较该模型中特定 RF 消融设备的性能与其他三种生物模型的性能。
使用新鲜牛肝创建三种体外模型：血液灌注、Krebs-Henseleit（KH）溶液灌注和非灌注。灌注模型通过门静脉与心肺机相连，并在生理条件下用肝素化的自体血液或 KH 溶液灌注。6 头猪用作体内肝模型。在所有模型中，使用簇状电极和 RF 消融系统以阻抗模式运行 12 分钟。对消融的肝脏进行切片，获得长轴和短轴的消融测量值，并进行统计分析。
在 14 个、6 个、5 个和 6 个血液灌注牛肝、KH 溶液灌注牛肝、非灌注牛肝和体内猪肝中分别进行了 39、23、17 和 12 次消融。在切割标本上，血液灌注肝脏中的消融区域平均直径为 4.00cm（95%CI，3.88-4.13），KH 溶液灌注肝脏中的为 4.34cm（95%CI，4.14-4.50），非灌注肝脏中的为 4.67cm（95%CI，4.50-4.83），体内猪肝中的为 3.56cm（95%CI，3.26-3.83）。在所有模型中，消融区域直径呈正态分布。
在体外血液灌注牛肝模型中，与 KH 溶液灌注或非灌注牛肝中产生的病变相比，RF 消融设备产生的消融区域大小更接近体内猪肝中的大小。
