Han K, Du S S, Wang H, Qiao J J, Zhang X, Wang P, Shen F H
School of Public Health, North China University of Science and Technology, Coal Mine Health and Safety Laboratory of Hebei Province, TangShan 063210, China.
Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi. 2021 Jan 20;39(1):17-19. doi: 10.3760/cma.j.cn121094-20190417-00155.
To identify the differential expression of Peroxiredoxin 4 (PRDX4) in alveolar macrophages (AMs) from patients with silicosis in different phases using Western blot. From June 2017 to June 2018, Twelve pneumoconiosis patients in the pneumoconiosis Department of Beidaihe Sanitarium were selected by random sampling. A msong them, there were 4 groups, that was lung with dust, silicosis with grade one, silicosis with grade two, silicosis with grade three. There were 3 persons in each group, a total of 12. AMs was obtained by filtration and centrifugation. The intracellular protein was extracted and PRDX4 was detected by using Western blot method. It results showed that PRDX4 was expressed in AMs in 4 groups; with the increase of fibrosis, the average relative expression of PRDX4 in AMs was 0.258±0.026, 0.214±0.012, 0.180±0.004, 0.165±0.008. The highest expression level was in the lung with dust group, and the lowest was in the silicosis with grade three group. The difference of the expression level of PRDX4 protein in AMs between groups was statistically significant (<0.05) . This experiment verified that PRDX4 expressed differentially in AMs in patients with silicosis. With the development of silicosis, PRDX4 expression in AMs reduced significantly.
采用蛋白质免疫印迹法检测不同期矽肺患者肺泡巨噬细胞（AMs）中过氧化物酶体增殖物激活受体4（PRDX4）的差异表达。2017年6月至2018年6月，随机抽取北戴河疗养院尘肺科12例尘肺患者。其中分为4组，即尘肺组、一期矽肺组、二期矽肺组、三期矽肺组。每组3人，共12人。通过过滤和离心获取AMs。提取细胞内蛋白质，采用蛋白质免疫印迹法检测PRDX4。结果显示，4组AMs中均有PRDX4表达；随着纤维化程度加重，AMs中PRDX4的平均相对表达量分别为0.258±0.026、0.214±0.012、0.180±0.004、0.165±0.008。尘肺组表达水平最高，三期矽肺组最低。各组AMs中PRDX4蛋白表达水平差异有统计学意义（<0.05）。本实验证实PRDX4在矽肺患者AMs中表达存在差异。随着矽肺病情发展，AMs中PRDX4表达明显降低。
